Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus

Applied and Environmental Microbiology
M Andrea Azcarate-PerilTodd R Klaenhammer

Abstract

Oxalic acid is found in dietary sources (such as coffee, tea, and chocolate) or is produced by the intestinal microflora from metabolic precursors, like ascorbic acid. In the human intestine, oxalate may combine with calcium, sodium, magnesium, or potassium to form less soluble salts, which can cause pathological disorders such as hyperoxaluria, urolithiasis, and renal failure in humans. In this study, an operon containing genes homologous to a formyl coenzyme A transferase gene (frc) and an oxalyl coenzyme A decarboxylase gene (oxc) was identified in the genome of the probiotic bacterium Lactobacillus acidophilus. Physiological analysis of a mutant harboring a deleted version of the frc gene confirmed that frc expression specifically improves survival in the presence of oxalic acid at pH 3.5 compared with the survival of the wild-type strain. Moreover, the frc mutant was unable to degrade oxalate. These genes, which have not previously been described in lactobacilli, appear to be responsible for oxalate degradation in this organism. Transcriptional analysis using cDNA microarrays and reverse transcription-quantitative PCR revealed that mildly acidic conditions were a prerequisite for frc and oxc transcription. As a consequence...Continue Reading

References

Jan 1, 1978·Scandinavian Journal of Gastroenterology·H AnderssonL Hultén
Jun 1, 1983·Applied and Environmental Microbiology·S F Barefoot, T R Klaenhammer
Jan 1, 1996·International Journal of Urology : Official Journal of the Japanese Urological Association·H ItoT Hara
May 1, 1996·FEMS Microbiology Letters·D C WalkerT R Klaenhammer
Mar 7, 1998·Journal of Molecular Evolution·K B Axelsen, M G Palmgren
Jun 1, 2000·Microbiology and Immunology·S HokamaY Ogawa
Nov 9, 2000·World Journal of Surgery·Y OgawaT Hatano
Mar 10, 2001·Journal of Dairy Science·M E Sanders, T R Klaenhammer
Dec 26, 2001·FEBS Letters·J Heider
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·A GiuliettiC Mathieu
Jul 31, 2002·Applied and Environmental Microbiology·Sylvia H DuncanColin S Stewart
Feb 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Michiel KleerebezemRoland J Siezen
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·R David PridmoreMark A Schell
Jun 9, 2004·Applied and Environmental Microbiology·Gerald W Tannock
Sep 4, 2004·Applied and Environmental Microbiology·Federica FedericiPatrizia Brigidi
Sep 10, 2004·Postgraduate Medical Journal·H S Gill, F Guarner
Jan 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Eric AltermannTodd R Klaenhammer
Feb 18, 2005·European Urology·C E Hoesl, J E Altwein
Sep 3, 2005·Nature Reviews. Microbiology·Christopher M Thomas, Kaare M Nielsen
Oct 6, 2005·Applied and Environmental Microbiology·M Andrea Azcarate-PerilTodd R Klaenhammer

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Citations

Mar 13, 2010·Urological Research·Joseph Okombo, Michael Liebman
Feb 10, 2011·The British Journal of Nutrition·Jelena ŠtšepetovaMarika Mikelsaar
Jun 22, 2010·Applied and Environmental Microbiology·Yong Jun Goh, Todd R Klaenhammer
Jun 10, 2008·Applied and Environmental Microbiology·M Andrea Azcarate-PerilTodd R Klaenhammer
Jul 28, 2009·Applied and Environmental Microbiology·Erika A Pfeiler, Todd R Klaenhammer
Aug 13, 2013·PloS One·Elwood A MullinsT Joseph Kappock
Jan 11, 2016·World Journal of Microbiology & Biotechnology·Vincent HervéPilar Junier
Apr 24, 2010·Veterinary Microbiology·Satoko KawajiRichard J Whittington
Apr 25, 2008·Journal of Plant Physiology·Dong-Gi LeeByung-Hyun Lee
Nov 22, 2008·BJU International·Siddharth SivaMahendra Bhandari
Mar 8, 2011·Microbial Biotechnology·Tri DuongTodd R Klaenhammer
Jan 22, 2010·Molecular Ecology·C MonardF Binet
Jul 4, 2009·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Anna-Liisa PrangliRaivo Uibo
Mar 25, 2014·Journal of Applied Microbiology·B KullinS J Reid
Mar 16, 2013·FEMS Microbiology Reviews·Kurt Selle, Todd R Klaenhammer
Feb 24, 2009·Journal of Dairy Science·M A Azcarate-PerilT R Klaenhammer
Jun 19, 2017·Microbial Pathogenesis·Humaira SadafSyed Waqas Hassan
May 12, 2018·Journal of the American Society of Nephrology : JASN·Gregory E TasianMichelle R Denburg
Aug 21, 2007·Clinical Journal of the American Society of Nephrology : CJASN·David S GoldfarbJohn R Asplin
May 31, 2019·Current Opinion in Nephrology and Hypertension·Menghan Liu, Lama Nazzal
May 31, 2019·Current Opinion in Nephrology and Hypertension·Shivam Joshi, David S Goldfarb
May 10, 2020·Journal of the American Society of Nephrology : JASN·Michelle R DenburgGregory E Tasian
Jun 9, 2018·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Shinji KatoAtsushi Yokota

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