Transcriptional and functional differences in stem cell populations isolated from extraocular and limb muscles.

Physiological Genomics
Eugenia C Pacheco-PinedoTejvir S Khurana

Abstract

The extraocular muscles (EOMs) are a distinct muscle group that displays an array of unique contractile, structural, and regenerative properties. They also have differential sensitivity to certain diseases and are enigmatically spared in Duchenne muscular dystrophy (DMD). The EOMs are so distinct from other skeletal muscles that the term "allotype" has been coined to highlight EOM group-specific properties. We hypothesized that increased and distinct stem cells may underlie the continual myogenesis noted in EOM. The side population (SP) stem cells were isolated and studied. EOMs had 15x higher SP cell content compared with limb muscles. Expression profiling revealed 348 transcripts that define the EOM-SP transcriptome. Over 92% of transcripts were SP specific, because they were absent in previous whole muscle microarray studies. Cultured EOM-SP cells revealed superior in vitro proliferative capacity. Finally, assays of the committed progenitors or satellite cells performed on myofibers isolated from EOM and limb muscles independently validated the increased proliferative capacity of these muscles. We suggest a model in which unique EOM stem cells contribute to the continual myogenesis noted in EOM and consistent with a role for...Continue Reading

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Citations

Jul 18, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Branka Stirn KranjcIda Erzen
Feb 11, 2010·Physiological Genomics·Ulrike Zeiger, Tejvir S Khurana
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Nov 8, 2017·Current Ophthalmology Reports·Mayank VermaLinda K McLoon
Feb 20, 2019·Bio-protocol·Amit SharmaBithiah Grace Jaganathan

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