PMID: 9164845May 15, 1997Paper

Transcriptional and post-transcriptional mechanisms can regulate cell-specific expression of the human Pi-class glutathione S-transferase gene

The Biochemical Journal
G J MoffatC R Wolf

Abstract

Previous studies from this laboratory have identified transcriptional mechanisms that are utilized to increase expression of the human glutathione S-transferase gene GSTP1 in a multidrug-resistant derivative (VCREMS) of the human mammary carcinoma cell line MCF7 [Moffat, McLaren and Wolf (1994) J. Biol. Chem. 269, 16397-16402]. The data presented here provide strong evidence that post-transcriptional mechanisms can also play an important role in determining cell-specific expression of the GSTP1 gene. GSTP1 mRNA levels were shown to be elevated 3.1-fold in the human bladder carcinoma cell line EJ compared with VCREMS cells. Despite this observation, transient transfection assays revealed a decreased rate of GSTP1 promoter activity in EJ cells. Indeed, GSTP1 transcriptional repressor activity, mediated by a region located between nucleotides -105 and -86 (as we have previously described in MCF7 cells), was observed in EJ cells. However, in contrast with our results in MCF7 cells, the EJ repressor activity did not displace the essential nuclear complex bound to the C1 promoter element (-73 to -54) in vitro. In addition, competition experiments indicated that an AP-1-like protein is an integral component of the C1-bound complex in ...Continue Reading

Citations

Aug 28, 2015·The Journal of Pharmacology and Experimental Therapeutics·David J McGarryColin J Henderson
Mar 24, 2011·Drug Metabolism Reviews·Colin J Henderson, C Roland Wolf
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·C J HendersonC R Wolf
Jun 2, 2007·Expert Opinion on Drug Metabolism & Toxicology·Philip G Board
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiu Jun WangC Roland Wolf
Aug 11, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·John P FruehaufRicardo Parker
Jun 18, 2014·Cancer Research·Colin J HendersonC Roland Wolf
Jul 29, 1998·Chemico-biological Interactions·C J HendersonC R Wolf

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