PMID: 2495271Apr 1, 1989Paper

Transcriptional and posttranscriptional control of the Bacillus subtilis succinate dehydrogenase operon

Journal of Bacteriology
L MelinA von Gabain

Abstract

The amount of succinate dehydrogenase (SDH) in Bacillus subtilis varies with growth conditions. In this work we studied the steady-state level and the rate of decay of B. subtilis sdh mRNA under different growth conditions. In exponentially growing cells, the steady-state level of sdh mRNA was severalfold lower when glucose was present compared with growth without glucose, whereas the rate of decay of sdh mRNA was the same with and without glucose. Thus, glucose repression seems to act by decreasing sdh mRNA synthesis. When the bacteria entered the stationary phase, the steady-state level of sdh mRNA dropped about sixfold. At the same time, sdh mRNA half-life decreased from 2.6 to 0.4 min. This result indicates that transcription of the sdh operon is initiated at the same rate in exponentially growing and in stationary-phase cells. The start point of the sdh transcripts, as measured by primer extension, was the same under all conditions studied, suggesting that the sdh operon is solely controlled by the previously identified sigma 43-like promoter. The increase of SDH activity in stationary phase may be explained by reduced dilution of the SDH proteins as a result of the retarded growth rate. We suggest that enhanced degradatio...Continue Reading

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Citations

Jul 29, 2003·Molecular Genetics and Genomics : MGG·G HambraeusL Hederstedt
Mar 1, 1993·Molecular Microbiology·J F DiMari, D H Bechhofer
May 20, 2006·Antonie van Leeuwenhoek·Weiwen ZhangFred J Brockman
Apr 24, 1996·Molecular & General Genetics : MGG·R Allmansberger
Dec 15, 1992·FEMS Microbiology Letters·C von Wachenfeldt, L Hederstedt
Mar 10, 2001·Environmental Microbiology·K Takayama, S Kjelleberg
Oct 6, 2020·Frontiers in Microbiology·Diego A Vargas-Blanco, Scarlet S Shell
Oct 23, 2009·Journal of Proteome Research·Maria BendzPeter James
Aug 1, 1994·Journal of Bacteriology·S Jin, A L Sonenshein

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