Transcriptional and posttranscriptional regulation of interstitial collagenase by platelet-derived growth factor BB in bone cell cultures

Endocrinology
S VargheseE Canalis

Abstract

Platelet-derived growth factor (PDGF), a bone cell mitogen, stimulates bone collagen degradation and does not enhance bone matrix apposition rates. The mechanism of the effect on collagen degradation is unknown, and it could involve changes in interstitial collagenase synthesis. We tested the effects of PDGF on interstitial collagenase expression in cultures of osteoblast-enriched cells from fetal rat calvariae (Ob cells). After 4-8 h of treatment, PDGF BB at 0.3 nM increased steady state collagenase messenger RNA (mRNA), whereas PDGF AA had no effect. The effect of PDGF BB on collagenase transcripts was dose dependent. PDGF BB increased the levels of immunoreactive collagenase after 6 h, whereas the levels were decreased after 16 h. Stimulation of collagenase mRNA by PDGF BB was dependent on de novo protein synthesis and activation of protein kinase C. PDGF BB prolonged the half-life of collagenase mRNA in transcriptionally arrested cells. PDGF BB initially increased and subsequently decreased the rate of collagenase gene transcription and the levels of collagenase heterogeneous nuclear RNA. In conclusion, PDGF BB regulates interstitial collagenase in Ob cells by transcriptional and posttranscriptional mechanisms, and this eff...Continue Reading

Citations

Apr 16, 1998·Journal of Hepatology·A CemborainJ Prieto
Nov 10, 2009·Matrix Biology : Journal of the International Society for Matrix Biology·Pranali PatelPeter J Margetts
Sep 19, 1997·Journal of Dental Research·W V GiannobileS E Lynch
Oct 21, 1999·Journal of Cellular Physiology·S K WinchesterN C Partridge
Jul 1, 1997·Journal of Cellular Biochemistry·B Gabbitas, E Canalis
Nov 19, 2003·Journal of Cellular Biochemistry·Samuel Varghese, Ernesto Canalis
Nov 5, 1997·Journal of Cellular Biochemistry·S RydzielE Canalis
Oct 18, 2007·Journal of Cellular Physiology·Yung-Cheng ChiuChih-Hsin Tang
Sep 14, 1999·The American Journal of Physiology·R C PereiraE Canalis
Mar 10, 1999·The American Journal of Physiology·S VargheseE Canalis

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