PMID: 6160039Sep 1, 1980Paper

Transcriptional and translational control of glucose-stimulated (pro)insulin biosynthesis

European Journal of Biochemistry
H JahrH Zühlke

Abstract

Islets of Langerhans were isolated from the pancreata of fed or 48-h-fasted Wistar rats. The islets were incubated with either [3H]leucine of [3H]uridine. Inhibition of RNA synthesis by actinomycin D or by alpha-amanitin for 4 h had no influence on the (pro)insulin biosynthesis of isolated islets of fed rats. The (pro)insulin biosynthesis was not inhibited after two days incubation of islets of fed rats with alpha-amanitin either. Incorporation of labelled uridine into total RNA for 3 h was stimulated by glucose in islets of fasted, but not of fed rats. Therefore, it was concluded that transcriptional control does not participate, even for longer periods than believed previously, in acute regulation of (pro)insulin biosynthesis of islets isolated fed rats. Despite the strong and preferential stimulation of (pro)insulin biosynthesis of islets of fed rats by glucose the radioactivity of the [3H]uridine-labelled polysomes active in proinsulin synthesis remained unchanged. To interprete these experimental data we suggest that glucose triggers the transformation of a translationally inactive form of pre-proinsulin mRNA to a translationally active form.

References

Aug 15, 1978·The Biochemical Journal·S J AshcroftC J Hedeskov
Oct 15, 1978·The Biochemical Journal·K JainJ Logothetopoulos
Aug 1, 1979·Molecular and Cellular Endocrinology·R P Munjaal, G F Saunders
May 31, 1979·Molecular Biology Reports·T MartinP Shaw
Apr 1, 1978·Developmental Biology·J F KaumeyerR A Raff
Jan 1, 1976·Annual Review of Biochemistry·H F Lodish
Nov 11, 1977·Science·P T Lomedico, G F Saunders
Aug 30, 1975·Molecular and Cellular Biochemistry·K Hemminki
Apr 1, 1976·Archives of Ophthalmology·H Schatz
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·E A Zylber, S Penman
Nov 1, 1974·Canadian Journal of Biochemistry·C L Hew, C C Yip
Dec 5, 1974·European Journal of Clinical Investigation·H StakebergT Scherstén
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M Jacobs-Lorena, C Baglioni
Feb 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M A Permutt, D M Kipnis
Aug 1, 1973·Endocrinology·J D ReinblattA D Kenny
Sep 1, 1969·Canadian Journal of Physiology and Pharmacology·B J Lin, R E Haist
Jun 1, 1970·Biochimica Et Biophysica Acta·G E Morris, A Korner
Jan 3, 1980·Nature·N Itoh, H Okamoto
Oct 5, 1970·FEBS Letters·G E. Morris, A Korner
Jun 1, 1965·General and Comparative Endocrinology·S MOSKALEWSKI

❮ Previous
Next ❯

Citations

Nov 1, 1982·Molecular and Cellular Endocrinology·K D KohnertD Schröder
Oct 1, 1983·Molecular and Cellular Endocrinology·D SchröderH Zühlke
May 3, 1984·The New England Journal of Medicine·D C RobbinsA H Rubenstein
Mar 2, 2011·The Journal of Biological Chemistry·Shardul D KulkarniVasudevan Seshadri
May 1, 1993·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·D SchröderS Schmidt
Aug 10, 2007·FEBS Letters·Bhavana MuralidharanVasudevan Seshadri
Feb 1, 1982·General and Comparative Endocrinology·A Huth, T A Rapoport
Mar 1, 1992·Diabetes Research and Clinical Practice·J TakahashiK Shima
Aug 15, 2006·Biochemical and Biophysical Research Communications·Andrew J SmithAsipu Sivaprasadarao
Dec 12, 2013·Molecular Metabolism·Maria Grazia Magro, Michele Solimena
Mar 6, 2012·Molecular Cell·Eun Kyung LeeMyriam Gorospe
Sep 1, 2000·Seminars in Cell & Developmental Biology·K A Goodge, J C Hutton
Oct 16, 2015·PLoS Biology·Justin R HasslerRandal J Kaufman
Mar 5, 2003·Pharmacological Reviews·Máire E Doyle, Josephine M Egan
Jan 1, 1988·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·F WohlrabL Cossel
Sep 8, 2020·Diabetologia·Jovana VasiljevićMichele Solimena
Jun 30, 1982·Biochemical and Biophysical Research Communications·J Brunstedt, S J Chan
Dec 30, 1986·Biochemical and Biophysical Research Communications·H FukumotoH Imura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.