Transcriptional and translational regulation of the marRAB multiple antibiotic resistance operon in Escherichia coli

Molecular Microbiology
Robert G Martin, Judah L Rosner

Abstract

The marRAB multiple antibiotic resistance operon of Escherichia coli is autorepressed by MarR. MarR binds to two palindromic sequences in vitro: site I lies between and overlaps the -35 and -10 hexamers for RNA polymerase binding; site II lies between the transcription start site and the GTG initiation codon of marR. To assess the importance of these sites in vivo, the effects of mutant sites on transcription were analysed using fusions to lacZ in the presence and absence of wild-type MarR. When both sites were wild type, transcription in the derepressed marR-deleted strain was 19-fold that of the wild-type strain; when only site I or site II was wild type, this ratio was reduced to 4.3- and 2.6-fold, respectively, showing that full repression requires both sites, but some repression can occur at one site independently of the other. Translational fusions of the wild-type promoter to lacZ demonstrated that marR translation proceeds at only 4.5% of the transcription rate. Analysis of translational fusions with mutant leader sequences demonstrated that the principal reason for inefficient translation is a weak Shine-Dalgarno (SD) sequence, AGG(G). Although the SD sequence is located within the potential stem-loop structure of site...Continue Reading

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Citations

Mar 24, 2007·Molecular Plant-microbe Interactions : MPMI·Ramani S RaviralaMichael J D San Francisco
Mar 15, 2011·Journal of Bacteriology·Lon M Chubiz, Christopher V Rao
Aug 7, 2007·Journal of Bacteriology·Gabriella FiorentinoSimonetta Bartolucci
Jan 8, 2015·Biophysical Journal·Javier Garcia-Bernardo, Mary J Dunlop
Oct 9, 2015·Biophysical Journal·Mahendra Kumar PrajapatSupreet Saini
Mar 22, 2008·FEMS Microbiology Reviews·María Dolores RoldánConrado Moreno-Vivián
Dec 5, 2008·FEMS Microbiology Reviews·Jose Luis MartinezMaría Blanca Sánchez
Dec 19, 2009·PLoS Computational Biology·Michael E WallRobert G Martin
Nov 1, 2016·Scientific Reports·Guillermo RodrigoJuan F Poyatos
Mar 11, 2017·Scientific Reports·Tiebin WangMary J Dunlop
Sep 30, 2017·The Journal of Antimicrobial Chemotherapy·Lisa Praski AlzrigatDiarmaid Hughes
Apr 22, 2016·Molecular BioSystems·Kirti Jain, Supreet Saini
Oct 12, 2017·NPJ Systems Biology and Applications·Guillermo RodrigoJuan F Poyatos
Jan 16, 2016·PloS One·Sophie J WeissRyan T Gill
Nov 26, 2020·International Journal of Medical Microbiology : IJMM·Xichun PanHong Zhou
Aug 1, 2009·EcoSal Plus·Akira Ishihama

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