Transcriptional and Translational Relationship in Environmental Stress: RNAseq and ITRAQ Proteomic Analysis Between Sexually Reproducing and Parthenogenetic Females in Moina micrura

Frontiers in Physiology
Jingyi JiaGuangfu Hu

Abstract

Moina micrura is a kind of small-bodied water flea within the family Moinidae. Similar to Daphnia, M. micrura could also switch its reproduction mode from parthenogenetic female (PF) to sexual female (SF) to adapt to the external environment. To uncover the mechanisms of reproductive switching in M. micrura, we used both RNA-Seq and iTRAQ analyses to investigate the differentially expressed genes (DEGs) and their protein products between SF and PF in M. micrura. A total of 1665 DEGs (702 up-regulated, 963 down-regulated) and 600 differentially expressed proteins (DEPs) (102 up-regulated, 498 down-regulated) were detected in SF. Correlation analyses indicated that 31 genes were expressed significantly differentially at both transcriptomic and proteomic levels, including 15 up-regulated genes and 16 down-regulated genes in SF. Meanwhile, our data also showed that 528 DEPs have discordant expression at transcript level, implying post-transcriptional (including translational) regulation. These top up-regulated genes and their protein products in SF were mainly grouped into the globin-related family, vitellogenin-related family, cuticle-related family, Hsp-related family and methyltransferases-related family, which were all involved...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R KlemenzA Aoyama
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·P BaldiM A McClure
Mar 1, 1996·Insect Biochemistry and Molecular Biology·J LiB M Christensen
Nov 15, 2001·Brazilian Journal of Biology = Revista Brasleira De Biologia·N E RojasO Rocha
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 9, 2003·The Journal of Biological Chemistry·Marc SchmidtThorsten Burmester
Mar 11, 2004·The Journal of Experimental Biology·Kevin Larade, Kenneth B Storey
Mar 17, 2005·Cellular and Molecular Life Sciences : CMLS·M P Mayer, B Bukau
Feb 15, 1929·Proceedings of the National Academy of Sciences of the United States of America·A M Banta, L A Brown
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Sep 15, 2006·Proteomics·Sixue Chen, Alice C Harmon
Dec 1, 2006·Apoptosis : an International Journal on Programmed Cell Death·Szabolcs BellyeiFerenc Gallyas
Feb 6, 2007·European Journal of Cell Biology·Szabolcs BellyeiFerenc Gallyas
Nov 22, 2007·Environment International·Valerio MatozzoChristian Blaise
Feb 27, 2008·BMC Bioinformatics·Kyung In Kim, Mark A van de Wiel
Mar 3, 2009·Environment International·Michiel B VandegehuchteColin R Janssen
May 27, 2009·Molecular Genetics and Genomics : MGG·Xiaoqian XuYunlong Zhao
Sep 26, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Sheng LiDavid W Borst
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Apr 13, 2010·Acta Biochimica Et Biophysica Sinica·Jie LanYong Zhang
May 17, 2011·Nature Biotechnology·Manfred G GrabherrAviv Regev
May 11, 2012·PloS One·Tamar SztalPhillip J Daborn
Mar 13, 2014·Development Genes and Evolution·Ajing LiuYunlong Zhao
Aug 4, 2015·The FEBS Journal·Gerald A LeBlanc, Elizabeth K Medlock

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Datasets Mentioned

BETA
SRP136759
IPX0001186000

Methods Mentioned

BETA
proteomic profiling
RNA-Seq
PCR
electrophoresis
GTPase
environmental stresses
environmental stress

Software Mentioned

Sickle
SPSS
WEGO
edgeR
Blastx
R Bioconductor package
Proteome Discoverer
R
Blast2GO ( Gene Ontology )
QuantStudio TM Real - Time PCR

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