PMID: 8955403Dec 1, 1996Paper

Transcriptional attenuation of the Bacillus subtilis pyr operon by the PyrR regulatory protein and uridine nucleotides in vitro

Journal of Bacteriology
Y Lu, Robert L Switzer

Abstract

Transcriptional attenuation of the pyrimidine biosynthetic (pyr) operon from Bacillus subtilis was reconstituted with an in vitro system that consisted of pyr DNA templates, B. subtilis RNA polymerase, four ribonucleoside triphosphates, and the purified B. subtilis PyrR regulatory protein. The templates used each specified one of the three known attenuation regions of the pyr operon. Runoff (read-though) and terminated transcripts of the predicted lengths were the only major products synthesized. Transcription of the template that specifies the 5' leader attenuation region of the operon was examined in detail. Termination of transcription at the attenuator was strongly promoted by the combination of PyrR plus UMP. The concentration of UMP required for half-maximal effect was 2.5 microM. UTP also promoted termination in the presence of PyrR, but concentrations 10-fold higher than UMP were required; UDP was only effective at 100 times the concentration of UMP. Other pyrimidine and purine metabolites tested did not affect termination. PRPP, which like UMP is a substrate for the uracil phosphoribosyltransferase activity of PyrR, antagonized UMP-dependent transcriptional termination, but uracil did not. Transcriptional attenuation b...Continue Reading

References

Aug 28, 1990·European Journal of Biochemistry·K ArnvigR L Switzer
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C L TurnboughJ P Donahue
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·M WengH Zalkin
Apr 20, 1995·Nature·A A AntsonP Gollnick

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Citations

Sep 1, 2005·Archives of Microbiology·Hesheng ZhangRobert L Switzer
Aug 15, 2018·Nucleic Acids Research·Jelle SlagerJan-Willem Veening
May 19, 2019·Annual Review of Microbiology·Paul BabitzkeTony Romeo
Dec 30, 1999·Journal of Bacteriology·C Yanofsky
Nov 5, 2003·Journal of Bacteriology·Casper Møller JørgensenJan Martinussen
Apr 9, 2001·Journal of Bacteriology·J MartinussenK Hammer
Jul 3, 2004·Journal of Bacteriology·Karen E BeenkenMark S Smeltzer
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Charles L Turnbough, Robert L Switzer
Jul 3, 2007·Journal of Bacteriology·Christopher J Fields, Robert L Switzer
Sep 6, 2007·BMC Genomics·Judith HübscherBrigitte Berger-Bächi

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