PMID: 7543474Aug 1, 1995Paper

Transcriptional autoregulation of the Salmonella typhimurium phoPQ operon

Journal of Bacteriology
Fernando C SonciniEduardo A Groisman

Abstract

The Salmonella typhimurium PhoP-PhoQ two-component regulatory system controls the expression of several genes, some of which are necessary for virulence. During a screening for PhoP-regulated genes, we identified the phoPQ operon as a PhoP-activated locus. beta-Galactosidase activity originating from phoPQ-lac transcriptional fusions required the presence of both the transcriptional regulator PhoP and its cognate sensor-kinase PhoQ. At low concentrations, PhoQ stimulated expression of phoPQ-lac transcriptional fusions. However, larger amounts of PhoQ protein without a concomitant increase in PhoP failed to activate phoPQ-lac fusions. Two different transcripts are produced from the phoPQ operon during exponential growth. These transcripts define two promoters: phoPp1, which requires both PhoP and PhoQ for activity and which is environmentally regulated, and phoPp2, which remains active in the absence of PhoP and PhoQ but which is slightly stimulated by these proteins. The pattern of transcriptional autoregulation was also observed at the protein level with anti-PhoP antibodies. In sum, autoregulation of the phoPQ operon provides several levels of control for the PhoP-PhoQ regulon. First, environmental signals would stimulate Pho...Continue Reading

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Citations

Oct 28, 2005·Molecular Biology of the Cell·Natalia Martin-OrozcoSergio Grinstein
Oct 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tim Miyashiro, Mark Goulian
Aug 6, 2008·The Journal of Biological Chemistry·Haowei SongYixin Shi
Jun 5, 2010·Molecular Plant-microbe Interactions : MPMI·Xin DengXiaoyan Tang
Aug 4, 2012·Molecular Microbiology·Eun-Jin Lee, Eduardo A Groisman
Oct 2, 2013·Annual Review of Genetics·Eduardo A GroismanMauricio H Pontes
Aug 27, 2013·Journal of Bacteriology·Brandon L JutrasBrian Stevenson
Nov 22, 2013·Molecular Microbiology·Sun-Yang Park, Eduardo A Groisman
Nov 5, 2013·Applied and Environmental Microbiology·Hakdong ShinSangryeol Ryu
May 5, 2017·The Protein Journal·Mohd Fakharul Zaman Raja YahyaSaiful Anuar Karsani
Jun 3, 2018·The EMBO Journal·Jinki YeomEduardo A Groisman
Dec 5, 2018·Journal of Bacteriology·María F BallesterosMónica A Delgado
Aug 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jinki YeomEduardo A Groisman
Apr 19, 2003·The Journal of Biological Chemistry·María E CastelliEleonora García Vescovi
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Feb 6, 2008·FEMS Microbiology Letters·Silvana V SpinelliFernando C Soncini
Mar 21, 2009·PLoS Genetics·J Christian PerezEduardo A Groisman
Feb 20, 2010·PLoS Computational Biology·J Christian J Ray, Oleg A Igoshin
Mar 23, 2012·Molecular Microbiology·Igor ZwirEduardo A Groisman
May 8, 2013·Molecular Microbiology·John F May, Eduardo A Groisman
Sep 9, 2016·Annual Review of Microbiology·Eduardo A Groisman
Feb 11, 2018·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Lucas Bocchini Rodrigues AlvesAngelo Berchieri Junior
Mar 15, 2019·Emerging Microbes & Infections·Jie RenYu-Feng Yao
Mar 3, 2019·Applied and Environmental Microbiology·Jeongjoon Choi, Sangryeol Ryu
Jun 20, 1998·The Journal of Biological Chemistry·M J Crawford, D E Goldberg
May 16, 2000·The Journal of Biological Chemistry·M E CastelliF C Soncini
Apr 26, 2002·Molecular Microbiology·Sangpen Chamnongpol, Eduardo A Groisman
Dec 21, 2006·Molecular Microbiology·Cécile RangAnne-Béatrice Blanc-Potard
Jun 14, 2005·Molecular Microbiology·Jetta J E Bijlsma, Eduardo A Groisman

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