Transcriptional changes following restoration of SERCA2a levels in failing rat hearts

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Federica Del MonteRoger J Hajjar

Abstract

Heart failure is characterized at the cellular level by impaired contractility and abnormal Ca2+ homeostasis. We have previously shown that restoration of a key enzyme that controls intracellular Ca(2+) handling, the sarcoplasmic reticulum Ca2+ ATPase (SERCA2a), induces functional improvement in heart failure. We used high-density oligonucleotide arrays to explore the effects of gene transfer of SERCA2a on genetic reprogramming in a model of heart failure. A total of 1,300 transcripts were identified to be unmodified by the effect of virus alone. Of those, 251 transcripts were found to be up- or down-regulated upon failure. A total of 51 transcripts which were either up--(27) or down--(24) regulated in heart failure were normalized to the nonfailing levels by the restoration of SERCA2a by gene transfer. The microarray analysis identified new genes following SERCA2a restoration in heart failure, which will give us insights into their role in the normalization of multiple pathways within the failing cell.

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Mar 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Federica del MonteRoger J Hajjar

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Citations

Apr 5, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kazuo NiwanoMasahiko Kurabayashi
Oct 24, 2012·Nature Reviews. Cardiology·Changwon KhoRoger J Hajjar
Aug 14, 2010·Mediators of Inflammation·James V Gruber, Robert Holtz
Nov 26, 2010·Journal of Molecular and Cellular Cardiology·Judith K GwathmeyRoger J Hajjar
Oct 30, 2007·Biochemical and Biophysical Research Communications·Marcus C Schaub, Claus W Heizmann
Jun 8, 2012·Gene Therapy·J-S HulotR J Hajjar
Jun 14, 2020·International Journal of Molecular Sciences·Rodrigo Aguayo-Ortiz, L Michel Espinoza-Fonseca

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