Transcriptional changes in response to X chromosome dosage in the mouse: implications for X inactivation and the molecular basis of Turner Syndrome.

BMC Genomics
Alexandra M LopesNabeel A Affara

Abstract

X monosomic mice (39,XO) have a remarkably mild phenotype when compared to women with Turner syndrome (45,XO). The generally accepted hypothesis to explain this discrepancy is that the number of genes on the mouse X chromosome which escape X inactivation, and thus are expressed at higher levels in females, is very small. However this hypothesis has never been tested and only a small number of genes have been assayed for their X-inactivation status in the mouse. We performed a global expression analysis in four somatic tissues (brain, liver, kidney and muscle) of adult 40,XX and 39,XO mice using the Illumina Mouse WG-6 v1_1 Expression BeadChip and an extensive validation by quantitative real time PCR, in order to identify which genes are expressed from both X chromosomes. We identified several genes on the X chromosome which are overexpressed in XX females, including those previously reported as escaping X inactivation, as well as new candidates. However, the results obtained by microarray and qPCR were not fully concordant, illustrating the difficulty in ascertaining modest fold changes, such as those expected for genes escaping X inactivation. Remarkably, considerable variation was observed between tissues, suggesting that ina...Continue Reading

References

Jan 1, 1995·Trends in Genetics : TIG·C M Disteche
May 16, 1998·Human Molecular Genetics·A GreenfieldP Koopman
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·C M Disteche
Sep 26, 2000·Mammalian Genome : Official Journal of the International Mammalian Genome Society·K D Tsuchiya, H F Willard
May 25, 2002·Human Molecular Genetics·Jun XuArthur P Arnold
Apr 23, 2003·American Journal of Human Genetics·Anne FogliOdile Boespflug-Tanguy
Aug 6, 2003·Cytogenetic and Genome Research·C M DistecheK D Tsuchiya
May 18, 2004·Current Opinion in Cell Biology·Edith Heard
May 24, 2005·Nature Genetics·Adam S Raefski, Michael J O'Neill
Jun 28, 2005·The European Journal of Neuroscience·J XuA P Arnold
Oct 1, 2005·BMC Genetics·Catherine L Anderson, Carolyn J Brown
Dec 6, 2005·Gene Expression Patterns : GEP·Jun XuArthur P Arnold
Dec 13, 2005·Nature Genetics·Di Kim Nguyen, Christine M Disteche
Jul 11, 2006·Genome Research·Xia YangAldons J Lusis
Sep 5, 2006·Genomics·Zohreh TalebizadehMerlin G Butler
Mar 21, 2007·Behavioural Brain Research·Phoebe M Y Lynn, William Davies
Jan 8, 2008·Nucleic Acids Research·Simon M LinWarren A Kibbe
Apr 25, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jun XuChristine M Disteche
May 10, 2008·Bioinformatics·Pan DuSimon M Lin
May 30, 2008·Current Opinion in Cell Biology·Jennifer A Erwin, Jeannie T Lee
Aug 30, 2008·Annual Review of Genetics·Bernhard Payer, Jeannie T Lee
Jul 8, 2009·Genome Research·Hendrik MarksHendrik G Stunnenberg

❮ Previous
Next ❯

Citations

Jul 20, 2011·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Alexandra M LopesLaura Carrel
May 10, 2011·Human Genetics·Christine YangCarolyn J Brown
May 27, 2011·Human Genetics·Joel B BerletchChristine M Disteche
Dec 19, 2012·Reproduction, Fertility, and Development·R Laguna-BarrazaA Gutierrez-Adan
Aug 31, 2011·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·T RaudseppB P Chowdhary
Aug 10, 2013·Biology of Sex Differences·Xuqi ChenArthur P Arnold
Feb 12, 2013·Endocrinology·Xuqi ChenArthur P Arnold
May 17, 2012·PLoS Genetics·Xuqi ChenKaren Reue
Sep 16, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·L Christine TurtzoLouise D McCullough
Apr 16, 2014·Nature Reviews. Genetics·Xinxian DengChristine M Disteche
Feb 3, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Arthur P ArnoldShayna M Williams-Burris
May 9, 2012·Hormones and Behavior·Rodney M RitzelLouise D McCullough
Nov 15, 2011·Trends in Genetics : TIG·Arthur P Arnold
Jan 31, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Arthur P ArnoldKaren Reue
Dec 6, 2012·Genes, Brain, and Behavior·J T WolstenholmeS Bekiranov
Jul 25, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Ho-Su Sin, Satoshi H Namekawa
Apr 11, 2018·Clinical Epigenetics·Francisco Álvarez-Nava, Roberto Lanes
Feb 20, 2019·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Arthur P Arnold
Oct 21, 2018·The Biochemical Journal·Anthony FullamMartina Schröder
Aug 19, 2017·Biology of Sex Differences·Rachael J WernerNora Engel
Aug 20, 2020·Journal of Pediatric Endocrinology & Metabolism : JPEM·Rakhi MalhotraRajesh Khadgawat
Feb 18, 2017·Biology·Tamara Potapova, Gary J Gorbsky
Sep 26, 2019·Frontiers in Behavioral Neuroscience·Shawn M AardeJames D Jentsch
Jul 31, 2018·Current Diabetes Reports·Eric ChangKanakadurga Singer
Oct 16, 2020·Arteriosclerosis, Thrombosis, and Vascular Biology·Yasir AlSirajLisa A Cassis

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Datasets Mentioned

BETA
GSE13520

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

lumi normalisation
limma
GeneMapper
FatiGO
R package limma
BeadStudio
Ensembl
Blat
SNaPshot
R

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