PMID: 3320961Dec 23, 1987Paper

Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift

Nucleic Acids Research
M H HerruerR J Planta

Abstract

Shifting a yeast culture from an ethanol-based medium to a glucose-based medium causes a coordinate increase of the cellular levels of ribosomal protein mRNAs by about a factor 4 within 30 min. Making use of hybrid genes encompassing different portions of the 5'-flanking region of the L25-gene, we could show that the increase in mRNAs is a transcriptional event, mediated through DNA sequences upstream of the ribosomal protein (rp) genes. Further analysis revealed that sequence elements are involved that many rp-genes have in common and that previously were identified as transcription activation sites (RPG-boxes or UASrpg). Using appropriate deletion mutants of the fusion genes we could demonstrate that a single RPG-box is sufficient for the transcriptional upshift. In addition, both copy genes encoding rp28 which differ considerably in their extent of transcriptional activity, show the upshift effect in a proportional manner. Definite proof for the role of the UASrpg in nutritional regulation was obtained by examining the effect of a synthetic RPG-box on transcription.

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Citations

Mar 29, 2000·Cell Biology International·L K LarsenK Kristiansen
Apr 20, 1995·Molecular & General Genetics : MGG·J MooreK Kaiser
Jan 13, 2006·Current Genetics·Malte KleinschmidtGerhard H Braus
Sep 23, 2000·Molecular and Cellular Endocrinology·N SiennaB H Sells
May 4, 2000·Molecular Biology of the Cell·J J BonnerK Lea
Sep 12, 1988·Nucleic Acids Research·A ChambersS M Kingsman
Jul 11, 1989·Nucleic Acids Research·J C DorsmanL A Grivell
May 11, 1992·Nucleic Acids Research·P M GonçalvesR J Planta
Feb 7, 2001·Molecular and Cellular Biology·T L IoukR W Wozniak
Aug 6, 2011·Genome Biology·Charissa de BekkerHan A B Wösten
Mar 2, 2012·Genetics·Kristan K SteffenBrian K Kennedy
Sep 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Rosa M MarionErin K O'Shea
Dec 18, 2008·The FEBS Journal·Philip GroeneveldHans V Westerhoff
Mar 13, 2003·Archives of Microbiology·Jeanette BrejningNils Arneborg
Jul 20, 1999·Molecular and Cellular Biology·B LiJ R Warner
Dec 1, 1994·Yeast·J M Thevelein
Jun 1, 1989·Microbiological Reviews·J R Warner

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