Transcriptional diversity of DMRT1 (dsx- and mab3-related transcription factor 1) in human testis

Cell Research
Han Hua ChengRong Jia Zhou

Abstract

Recent advances in the evolutionary genetics of sex determination indicate that the only molecular similarity in sex determination found so far among phyla is between the fly doublesex, worm mab-3 and vertebrate DMRT1(dsx- and mab3-related transcription factor 1) /DMY genes. Each of these factors encodes a zinc-finger-like DNA-binding motif, DM domain. Insights into the evolution and functions of human DMRT1 gene could reveal evolutionary mechanisms of sexual development. Here we report the identification and characterization of multiple isoforms of human DMRT1 in the testis. These transcripts encode predicted proteins with 373, 275 and 175 amino acids and they were generated by alternative splicing at 3' region. Expression level of DMRT1a is higher than those of both DMRT1b and c, and the DMRT1c expression was the lowest in testis, based on comparisons of mean values from real-time fluorescent quantitative RT-PCR analysis. Both DMRT1b and c result from exonization of intronic sequences, including the exonization of an Alu element. A further search for Alu elements within the DMRT1 gene demonstrated that all 99 Alu elements are non-randomly distributed among the non-coding regions on both directions. These new characteristics o...Continue Reading

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Citations

Nov 23, 2006·Development Genes and Evolution·Yang ZhaoRongjia Zhou
Sep 8, 2010·Biochemical and Biophysical Research Communications·Lei ZhangRongjia Zhou
Oct 16, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Amine NaimiPascal Sourdaine
Dec 5, 2006·Biochemical and Biophysical Research Communications·Heng LuRongjia Zhou
Apr 5, 2016·Chemosphere·Clare Isabel Ming-Ch'eng AdamsBirthe V Kjellerup
Feb 23, 2011·Arquivos brasileiros de endocrinologia e metabologia·Maricilda Palandi de MelloGil Guerra-Júnior
Aug 7, 2007·Cytogenetic and Genome Research·C A SmithA H Sinclair

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