Transcriptional Dynamics of Grain Development in Barley (Hordeum vulgare L.)

International Journal of Molecular Sciences
Jianxin BianWeining Song

Abstract

Grain development, as a vital process in the crop's life cycle, is crucial for determining crop quality and yield. However, the molecular basis and regulatory network of barley grain development is not well understood at present. Here, we investigated the transcriptional dynamics of barley grain development through RNA sequencing at four developmental phases, including early prestorage phase (3 days post anthesis (DPA)), late prestorage or transition phase (8 DPA), early storage phase (13 DPA), and levels off stages (18 DPA). Transcriptome profiling found that pronounced shifts occurred in the abundance of transcripts involved in both primary and secondary metabolism during grain development. The transcripts' activity was decreased during maturation while the largest divergence was observed between the transitions from prestorage phase to storage phase, which coincided with the physiological changes. Furthermore, the transcription factors, hormone signal transduction-related as well as sugar-metabolism-related genes, were found to play a crucial role in barley grain development. Finally, 4771 RNA editing events were identified in these four development stages, and most of the RNA editing genes were preferentially expressed at t...Continue Reading

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Citations

Sep 6, 2019·International Journal of Molecular Sciences·Hongyou LiQingfu Chen
Mar 27, 2021·Scientific Data·Linda MilneCraig G Simpson
Jun 3, 2021·International Journal of Molecular Sciences·Sebastian GasparisAnna Nadolska-Orczyk
Oct 12, 2021·The Plant Journal : for Cell and Molecular Biology·Guangbin LuoAimin Zhang

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Datasets Mentioned

BETA
SUB2126402

Methods Mentioned

BETA
RNA-seq
chip
dissection
Illumina sequencing

Software Mentioned

R
- toolkit
KOBAS
Samtools
KAAS ( KEGG Automatic Annotation Server )
HISAT2
BLAST
iTAK
AgriGO
HTSeq

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