Transcriptional Dynamics of Hepatic Sinusoid-Associated Cells After Liver Injury.

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Mike K TerkelsenKim Ravnskjaer

Abstract

Hepatic sinusoidal cells are known actors in the fibrogenic response to injury. Activated hepatic stellate cells (HSCs), liver sinusoidal endothelial cells, and Kupffer cells are responsible for sinusoidal capillarization and perisinusoidal matrix deposition, impairing vascular exchange and heightening the risk of advanced fibrosis. While the overall pathogenesis is well understood, functional relations between cellular transitions during fibrogenesis are only beginning to be resolved. At single-cell resolution, we here explored the heterogeneity of individual cell types and dissected their transitions and crosstalk during fibrogenesis. We applied single-cell transcriptomics to map the heterogeneity of sinusoid-associated cells in healthy and injured livers and reconstructed the single-lineage HSC trajectory from pericyte to myofibroblast. Stratifying each sinusoidal cell population by activation state, we projected shifts in sinusoidal communication upon injury. Weighted gene correlation network analysis of the HSC trajectory led to the identification of core genes whose expression proved highly predictive of advanced fibrosis in patients with nonalcoholic steatohepatitis (NASH). Among the core members of the injury-repressed ...Continue Reading

References

Jul 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E WisseR S McCuskey
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Bin Zhang, Steve Horvath
Jan 30, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Christian MöllekenKai Stühler
Mar 13, 2012·The Journal of Clinical Investigation·Lin WangLaurie D DeLeve
Aug 6, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Cynthia A MoylanAnna Mae Diehl
Feb 26, 2014·Nature Reviews. Immunology·Antonella PellicoroJonathan A Fallowfield
Jan 13, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bianca M ArendtJohane P Allard
Jan 15, 2015·Journal of Gastroenterology and Hepatology·Elzafir ElsheikhZobair M Younossi
Sep 4, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Kenjiro Wake, Tetsuji Sato
May 7, 2016·Journal of Hepatology·Giusi MarroneJordi Gracia-Sancho
Jul 18, 2016·Journal of Hepatology·Johanne PoissonPierre-Emmanuel Rautou
Dec 3, 2016·The Journal of Cell Biology·Thomas NeillRenato V Iozzo
Jan 29, 2017·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Parambir S DulaiRohit Loomba
Feb 22, 2017·The Journal of Clinical Investigation·Cyrill GéraudSergij Goerdt
Sep 18, 2018·Nature Biotechnology·Keren Bahar HalpernShalev Itzkovitz
Oct 5, 2018·Nature·UNKNOWN Tabula Muris ConsortiumUNKNOWN Principal investigators
Nov 16, 2018·Nature·Roser Vento-TormoSarah A Teichmann
Dec 21, 2018·Nature Reviews. Gastroenterology & Hepatology·Jordi Gracia-SanchoAnabel Fernández-Iglesias
Feb 23, 2019·Scientific Reports·Ann-Britt MarcherKim Ravnskjaer

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Citations

Mar 22, 2021·Angiogenesis·Philipp-Sebastian KochHellmut G Augustin
May 28, 2021·JHEP Reports : Innovation in Hepatology·Nikolaj TorpAleksander Krag
May 27, 2021·Biomarker Insights·Susanne Gjørup SækmoseGrith Lykke Sorensen
Jun 6, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wu YangJunhao Hu
Nov 9, 2021·Frontiers in Medicine·Stefaan VerhulstLeo A van Grunsven

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Datasets Mentioned

BETA
GSE49541
GSE89632

Methods Mentioned

BETA
enzymatic dissociation
flow cytometry
Biopsies
Fluorescence
scRNAseq
biopsy
gastric bypass

Software Mentioned

Python
R
CellPhoneDB
Plvap

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