Transcriptional effects of PFOS in isolated hepatocytes from Atlantic salmon Salmo salar L

Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Anne Vatland KrøvelPål A Olsvik

Abstract

The main aim of the current in vitro experiment was to search for makers for PFOS exposure in isolated hepatocytes from Atlantic salmon Salmo salar, based on genes responding to PFOS exposure in other animals. Primary cell cultures of hepatocytes were exposed to four concentrations of PFOS (2.1-6.2-15.1-25.0 mg/L) for 24 and 48 h and the transcriptional levels of 12 genes encoding proteins known to respond to PFOS were quantified with real-time RT-PCR. The 12 examined genes were caspase 3B (apoptosis), GSH-Px and HSP70 (cellular stress), CYP1A, CYP3A, GST and UGT (P450 and phase II enzymes), acyl-CoA oxidase, PPARalpha, PPARbeta and PPARgamma (lipid metabolism) and Na(+)-K(+)-ATPase (ion regulation). Most of the studied genes responded in a dose-dependent manner to PFOS exposure, although the transcriptional differences in general where small with regard to fold change. Our results clearly suggest that PFOS exposure enhanced cellular stress in the examined cells, even though the exact mechanisms behind this stress remain unknown. The results from this in vitro experiment showed that genes known to be affected by PFOS exposure in other species also were induced in hepatocytes of Atlantic salmon, giving us the rationale to expand...Continue Reading

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Citations

Oct 7, 2014·Ecotoxicology and Environmental Safety·Srinithi MayilswamiRavi Naidu
Feb 26, 2016·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Reyna Cristina Collí-DuláRandy Lehr
Jan 25, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yanyan LiaoSijun Dong
Apr 5, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Anne Vatland KrøvelPål A Olsvik
Jan 14, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Liv SøftelandPål A Olsvik
Oct 31, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Anne Vatland KrøvelPål A Olsvik
Sep 15, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Tien-Sheng HuangBente E Torstensen
Dec 4, 2013·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Shaoli YangSong Qin
Jun 1, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I SovadinováK Schirmer
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Mar 5, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Xiongjie Shi, Bingsheng Zhou
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Nov 15, 2019·Environmental Toxicology and Chemistry·Linbao ZhangWengui Cai
Apr 14, 2020·Environmental Pollution·Yong-Zhe LiuZhong-Ze Fang

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