Transcriptional factor prolactin regulatory element-binding protein-mediated gene transcription of ABCA1 via 3',5'-cyclic adenosine-5'-monophosphate

Atherosclerosis
Y NishiuchiT Ishida

Abstract

Prolactin regulatory element-binding (PREB) protein is a transcription factor that regulates prolactin promoter activity in the rat anterior pituitary. The PREB protein is not only expressed in the anterior pituitary but also in the cardiovascular system, including vascular smooth muscle cells (SMCs). However, the role of PREB in SMCs is not clearly understood. The ATP-binding cassette transporter A1 (ABCA1) regulates lipid efflux from peripheral cells to apolipoproteins. In the present study, we have examined the role of PREB in regulating ABCA1 expression mediated by 3',5'-cyclic adenosine-5'-monophosphate (cAMP). PREB was expressed in the rats SMC line CRL-2018. ABCA1 expression was found to be regulated by cAMP, which stimulated the expression of PREB in a dose-dependent manner. Conversely, over-expression of PREB, which was induced by a PREB-expressing adenovirus, increased the expression of the ABCA1 protein in CRL-2018 cells. In addition, PREB stimulated the activity of the luciferase reporter protein that was under the control of the ABCA1 promoter. Chromatin immunoprecipitation assay showed that PREB mediates its transcriptional activity by directly binding to the ABCA1 promoter region. Finally, we used siRNA to inhibi...Continue Reading

References

Aug 1, 1990·The Journal of Clinical Investigation·A R Tall
Oct 13, 1998·Annals of Clinical Biochemistry·B ShahnazK Kawanishi
Mar 8, 2000·Genomics·C L Taylor ClellandT Lufkin
Jul 14, 2000·The Journal of Biological Chemistry·A E BortnickO L Francone
Aug 2, 2000·Mammalian Genome : Official Journal of the International Mammalian Genome Society·C L Taylor ClellandC Bancroft
Nov 22, 2000·The Journal of Clinical Investigation·A R Tall, N Wang
Aug 28, 2002·The Journal of Biological Chemistry·Raymond H SeeMichael R Hayden
Nov 28, 2002·Journal of Lipid Research·Bassam HaidarJacques Genest
Mar 22, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Hitomi ImachiToshihiko Ishida
Aug 2, 2003·Methods : a Companion to Methods in Enzymology·Virginia A SpencerJames R Davie
Sep 20, 2008·Biochemical and Biophysical Research Communications·Hitomi ImachiToshihiko Ishida

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Citations

Oct 30, 2016·Molecular and Cellular Endocrinology·Corneille Edgar OntsoukaChristiane Albrecht
Aug 22, 2018·American Journal of Physiology. Endocrinology and Metabolism·Kensaku FukunagaKoji Murao
Aug 23, 2018·Journal of Molecular Endocrinology·Seisuke SatoKoji Murao

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