Transcriptional Identification of Related Proteins in the Immune System of the Crayfish Procambarus clarkii

High-throughput
Gabina Calderón-RoseteLeonardo Rodríguez-Sosa

Abstract

The freshwater crayfish Procambarus clarkii is an animal model employed for physiological and immunological studies and is also of great economic importance in aquaculture. Although it is a species of easy husbandry, a high percentage of its production is lost annually as a result of infectious diseases. Currently, genetic information about the immune system of crustaceans is limited. Therefore, we used the abdominal nerve cord from P. clarkii to obtain its transcriptome using Next Generation Sequencing (NGS) to identify proteins that participate in the immune system. The reads were assembled de novo and consensus sequences with more than 3000 nucleotides were selected for analysis. The transcripts of the sequences of RNA were edited for annotation and sent to the GenBank database of the National Center for Biotechnology Information (NCBI). We made a list of accession numbers of the sequences which were organized by the putative role of the immune system pathway in which they participate. In this work, we report on 80 proteins identified from the transcriptome of crayfish related to the immune system, 74 of them being the first reported for P. clarkii. We hope that the knowledge of these sequences will contribute significantly ...Continue Reading

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Datasets Mentioned

BETA
PRJNA356499
PRJNA387410
MG910465
AGZ84432.1

Methods Mentioned

BETA
RNA-seq

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
Trinity
Galaxy
BLAST
FASTA
Mega
Prosite
Inchworm
Galaxy Web
ClustalW

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