Transcriptional induction of cyclooxygenase-2 in osteoblasts is involved in interleukin-6-induced osteoclast formation

Endocrinology
H TaiT Suda

Abstract

Interleukin-6 (IL-6) induces osteoclast-like cell (osteoclast) formation in a dose-dependent fashion in cocultures of mouse bone marrow cells and osteoblastic cells when soluble IL-6 receptor (sIL-6R) is present. Simultaneous treatment with submaximal doses of IL-1alpha and IL-6 with sIL-6R caused marked induction of osteoclast formation and PGE2 synthesis. These effects were suppressed by adding neutralizing antibodies against IL-1alpha or IL-6R and were totally abolished by adding nonsteroidal antiinflammatory drugs, such as indomethacin and a selective cyclooxygenase-2 (COX-2) inhibitor (NS398). In mouse osteoblastic cells, both IL-1alpha and IL-6 with sIL-6R markedly induced messenger RNA expression of COX-2, but not COX-1, as determined by Northern blot analysis, and luciferase activity in cells stably transfected with a COX-2 promoter-luciferase fusion construct. IL-6 and sIL-6R, when added separately, did not stimulate COX-2 messenger RNA expression. Simultaneous addition of IL-1alpha and IL-6 with sIL-6R to osteoblast cultures cooperatively induced transcription of COX-2, which was associated with a marked increase in COX activity measured by the conversion of arachidonic acid into PGE2. The increased PGE2 synthesis by ...Continue Reading

References

Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·W L XieD L Simmons
May 8, 1991·Biochimica Et Biophysica Acta·D L DeWitt
Sep 1, 1991·Immunology Letters·H SuzukiT Kishimoto
Feb 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T AkatsuT Suda
Nov 1, 1988·European Journal of Immunology·T HiranoM Feldmann
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R PacificiW A Peck
Sep 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C MiyauraY Ohsugi
Aug 1, 1995·The Journal of Clinical Investigation·H KawaguchiM M Hurley
Mar 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C C PilbeamH Kawaguchi
Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J R HarrisonC Pilbeam
Apr 1, 1994·The Journal of Clinical Investigation·G GirasoleS C Manolagas
May 1, 1994·The Journal of Clinical Investigation·R B KimbleR Pacifici
Nov 3, 1993·Biochimica Et Biophysica Acta·I KudoK Inoue
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T TamuraT Taga
Jan 28, 1994·Cell·T KishimotoS Akira
Mar 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SatoS I Murota

❮ Previous
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Citations

Mar 23, 2000·The Journal of Clinical Investigation·Y OkadaC C Pilbeam
Mar 20, 2008·The American Journal of Pathology·Sasanka RamanadhamJohn Turk
Jan 31, 2008·Journal of Clinical Biochemistry and Nutrition·Hidetaka IshinoToshikazu Yoshikawa
Jan 16, 2004·Archives of Pharmacal Research·Seong-Soo JooDo-Ik Lee
Jan 1, 1997·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·T Suda, C Miyaura
Aug 12, 2009·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·David Weldon
Oct 21, 2000·Mayo Clinic Proceedings·N S Buttar, K K Wang
Mar 14, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nancy S KriegerDavid A Bushinsky
Apr 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·X ZhangE M Schwarz
May 6, 2004·Brain Research·Hidemichi SuyamaOsafumi Yuge
Mar 26, 2009·Clinical Orthopaedics and Related Research·Sigbjorn DimmenJan Erik Madsen
Jan 26, 2007·Journal of Dental Research·S OgataK Shirasuna
Feb 24, 2009·Toxicologic Pathology·Zaher A Radi
Nov 25, 2003·Seminars in Dialysis·Nancy S KriegerKevin K Frick
Jun 17, 2004·Current Opinion in Nephrology and Hypertension·Nancy S KriegerDavid A Bushinsky
May 15, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Roger RezzonicoHeidy Schmid-Antomarchi
Jul 14, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y K MinC C Pilbeam
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Laura D CarboneStephen B Kritchevsky
Oct 31, 2006·Journal of Bone and Mineral Metabolism·Chwan-Li ShenXingjia Wang
Sep 5, 2009·Clinical Rheumatology·Shuhei OginoHideshige Moriya
Dec 31, 2004·Endocrinology·Manshan XuCarol Pilbeam
Jun 13, 2012·Journal of Tissue Engineering and Regenerative Medicine·X Z YanX F Walboomers
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Ashraf A RagabEdward M Greenfield
Jul 23, 2019·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Berna I AydoğanSevim Güllü
Dec 13, 2017·Oxidative Medicine and Cellular Longevity·Miguel Tofiño-VianMaria José Alcaraz
Jul 11, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Céline ColnotMelissa L Knothe Tate
Oct 3, 1999·Physiological Reviews·S NarumiyaF Ushikubi
Nov 13, 2001·American Journal of Physiology. Renal Physiology·D A BushinskyN S Krieger
Nov 30, 2000·American Journal of Physiology. Renal Physiology·N S KriegerD A Bushinsky

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