Transcriptional induction of multiple cytokines by human respiratory syncytial virus requires activation of NF-kappa B and is inhibited by sodium salicylate and aspirin

Virology
V BitkoS Barik

Abstract

Infection of the lung epithelial cell line A549 by respiratory syncytial virus (RSV) resulted in the elevated synthesis of multiple cellular cytokines, including a number of interleukins (ILs). Detailed studies of IL-11 induction revealed that it required infection by viable virus and involved a net increase in the steady state level of IL-11 mRNA. Nuclear run-on assays showed a direct effect of RSV on IL-11 gene transcription. Mutational analysis of the IL-11 promoter fused to a reporter luciferase gene demonstrated the requirement of a region 720 nucleotides upstream of the mRNA start site in the transcriptional induction of IL-11 by RSV. Two nearly identical 10-nucleotide-long sequences GGGGTCTCCC and GGGTCTCCCC in this region resembled the NF-kappa B consensus motif. Mutation of either sequence greatly reduced RSV-mediated induction of IL-11 promoter activity. NF-kappa B sites in IL-1 alpha, IL-6, and IL-8 promoters were also required for RSV-mediated induction of transcription of these promoters. Immunological studies and use of reporter gene constructs provided direct evidence for the activation and nuclear translocation of NF-kappa B by RSV. Sodium salicylate and aspirin, inhibitors of NF-kappa B activation, abolished tr...Continue Reading

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S R PaulD A Williams
Apr 25, 1986·Nucleic Acids Research·Y FurutaniS Nakamura
Nov 15, 1995·Genes & Development·I M VermaS Miyamoto
Apr 28, 1995·The Journal of Biological Chemistry·S NwakaH Holzer
Jan 1, 1995·Advances in Immunology·E B Kopp, S Ghosh
Jan 1, 1995·The Journal of Infectious Diseases·L J Anderson, C A Heilman
Feb 24, 1995·Cell·D Thanos, T Maniatis
Jan 1, 1994·Annual Review of Cell Biology·U SiebenlistK Brown
Jan 1, 1994·Annual Review of Immunology·R A Seder, W E Paul
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Aug 12, 1994·Science·E Kopp, S Ghosh
Dec 1, 1996·Molecular and Cellular Biology·R J PhillipsS Ghosh

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Citations

Jul 29, 2004·Viral Immunology·Subramaniam KrishnanRobert C Welliver
Sep 19, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Santanu Bose, Amiya K Banerjee
Jul 18, 2012·Antioxidants & Redox Signaling·Roberto P GarofaloAntonella Casola
Nov 20, 1998·Allergy·S L Johnston
Jan 15, 2011·Clinical Microbiology Reviews·Marjolaine VareilleNicolas Regamey
Jul 16, 2005·Clinical Microbiology Reviews·Peter J M Openshaw, John S Tregoning
Oct 20, 2006·Journal of Virology·Vira BitkoSailen Barik
Sep 19, 2000·Thorax·R J GryglewskiJ Swieş
Oct 2, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yashoda M HosakoteAntonella Casola
May 23, 2002·American Journal of Respiratory and Critical Care Medicine·Helene A HaeberleRoberto P Garofalo
Jul 17, 2003·The Journal of Clinical Investigation·Leon Iri KupferwasserArnold S Bayer
Aug 18, 2004·The Journal of Clinical Investigation·Margit A HuberThomas Wirth
Feb 11, 2005·Virology Journal·Xiaoyuan KongShyam S Mohapatra
Mar 31, 2009·Future Microbiology·Christine M OshanskyRalph A Tripp
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Santanu BoseAmiya K Banerjee
Jan 22, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R K BhatM B Hershenson
May 19, 2000·Microbes and Infection·A GarciaE Sontag
Feb 15, 2001·Expert Opinion on Investigational Drugs·G A Prince
Feb 13, 2004·FEBS Letters·Xiaoyuan KongShyam S Mohapatra
May 16, 2000·Chest·J W ChristmanT S Blackwell
Sep 28, 2006·Reviews in Medical Virology·S van Drunen Littel-van den HurkJ Kovacs-Nolan
Feb 7, 2006·The Journal of Surgical Research·Balraj SinghAnthony Lucci

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