Transcriptional inhibition of the inducible nitric oxide synthase gene by competitive binding of NF-kappa B/Rel proteins

Biochemical and Biophysical Research Communications
C E GoldringJ F Jeannin

Abstract

The activity of the inducible nitric oxide synthase enzyme (iNOS) is tightly controlled, partly at the transcriptional level. We find NF-kappa B/Rel activation (p50-p50 and p50-p65) in RAW 264.7 macrophages after lipopolysaccharide treatment and binding to both NF-kappa B sites in the mouse iNOS promoter. To delineate the importance of NF-kappa B/Rel in iNOS gene transcription, we used an unusually direct approach to try to improve on the antioxidant-treatment or reporter techniques, namely the depletion of NF-kappa B/Rel activity through the use of a phosphorothioate-modified oligonucleotide containing three copies of the NF-kappa B consensus sequence. The reduction in NF-kappa B/Rel activity (particularly that binding to the downstream of the two sites) was associated with a 50% reduction in NO output and a reduction in the quantity of the iNOS protein expressed. These results point to the probability that physiologically relevant NF-kappa B/Rel activators or repressors other than lipopolysaccharide might crucially affect the macrophage NO response.

Citations

Dec 12, 2001·British Journal of Pharmacology·R FriedlP G Spieckermann
Oct 10, 2012·Organic & Biomolecular Chemistry·Xufen YuDianqing Sun
Jun 15, 2006·Antioxidants & Redox Signaling·Tiziana PersichiniMarco Colasanti
Jun 23, 2012·BMC Complementary and Alternative Medicine·Koh TomimoriNaoki Mori
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·R M RaiA M Diehl
Mar 28, 2012·Bioscience, Biotechnology, and Biochemistry·Reiko MaedaTatsuji Sakamoto
Dec 8, 2004·Biochemical and Biophysical Research Communications·Yong Man Kim, Sang-Gi Paik
Jan 15, 1999·Molecular Neurobiology·M Colasanti, G Venturini
Mar 12, 2009·The Journal of Trauma·Jong Woong ParkHyon Seok Jang
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C E GoldringJ F Jeannin
Nov 10, 1995·The Journal of Biological Chemistry·M ColasantiH Suzuki
Oct 14, 2003·European Journal of Cardiovascular Prevention and Rehabilitation : Official Journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology·Volker AdamsRainer Hambrecht
Dec 18, 2001·American Journal of Physiology. Cell Physiology·Xingwu TengJohn D Catravas
Feb 15, 2001·American Journal of Physiology. Cell Physiology·E D Chan, D W Riches
Nov 9, 2018·International Journal of Molecular Sciences·Chia-Hung Yen, Hui-Hua Hsiao
Jul 18, 2000·Toxicology and Applied Pharmacology·K J ChoA S Chung
Jun 18, 1997·Biochemical and Biophysical Research Communications·J XuC Y Hsu
Jun 7, 1996·Biochimica Et Biophysica Acta·H SakuraiT Sugita
May 24, 2000·Brain Research Bulletin·M Colasanti, T Persichini
May 30, 1998·The Journal of Laboratory and Clinical Medicine·J M PrzybockiR E Billings

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.