Transcriptional landscape changes during human embryonic stem cell derivation

Molecular Human Reproduction
Sharat WarrierBjörn Heindryckx

Abstract

What are the transcriptional changes occurring during the human embryonic stem cell (hESC) derivation process, from the inner cell mass (ICM) to post-ICM intermediate stage (PICMI) to hESC stage, that have downstream effects on pluripotency states and differentiation? We reveal that although the PICMI is transcriptionally similar to the hESC profile and distinct from ICM, it exhibits upregulation of primordial germ cell (PGC) markers, dependence on leukemia inhibitory factor (LIF) signaling, upregulation of naïve pluripotency-specific signaling networks and appears to be an intermediate switching point from naïve to primed pluripotency. It is currently known that the PICMI exhibits markers of early and late-epiblast stage. It is suggested that hESCs acquire primed pluripotency features due to the upregulation of post-implantation genes in the PICMI which renders them predisposed towards differentiation cues. Despite this current knowledge, the transcriptional landscape changes during hESC derivation from ICM to hESC and the effect of PICMI on pluripotent state is still not well defined. To gain insight into the signaling mechanisms that may govern the ICM to PICMI to hESC transition, comparative RNA sequencing (RNA-seq) analysi...Continue Reading

References

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Nov 6, 1998·Science·J A ThomsonJ M Jones
May 20, 2004·Human Molecular Genetics·Anthony T DobsonRenee A Reijo Pera
Jun 29, 2007·Nature·I Gabrielle M BronsLudovic Vallier
Mar 26, 2008·Nature·Sanjay K SinghSadhan Majumder
Jun 20, 2008·Genome Research·Marcel E DingerJohn S Mattick
May 29, 2009·BMC Bioinformatics·Weijun LuoPeter J Woolf
Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Apr 2, 2010·Journal of Assisted Reproduction and Genetics·Ann A KiesslingDimitris Loutradis
Sep 3, 2010·Stem Cells and Development·Thomas O'LearyPetra De Sutter
Sep 8, 2010·Cell Research·Oscar H Ocaña, M Angela Nieto
Jan 5, 2011·PloS One·Eva Gonzalez-RocaHerbert Auer
Jul 14, 2011·Stem Cell Reviews and Reports·Qiang BaiJohn De Vos
Mar 1, 2012·Nature Biotechnology·Thomas O'LearyPetra De Sutter
Sep 8, 2012·Genome Research·Jennifer HarrowTim J Hubbard
Aug 13, 2013·Nature Structural & Molecular Biology·Liying YanFuchou Tang
Mar 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Carol B WareHannele Ruohola-Baker
Jul 20, 2014·Journal of Cell Science·Yong TangXiuchun Cindy Tian
Oct 29, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thorsten Boroviak, Jennifer Nichols
Jun 9, 2015·Cellular Reprogramming·Margot Van der JeughtBjörn Heindryckx
Jun 20, 2015·Human Reproduction Update·Margot Van der JeughtBjörn Heindryckx

❮ Previous
Next ❯

Citations

Mar 7, 2020·Human Reproduction Update·Mina PopovicBjörn Heindryckx

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.