Transcriptional landscape of bone marrow-derived very small embryonic-like stem cells during hypoxia.

Respiratory Research
Sina A GharibDavid Gozal

Abstract

Hypoxia is a ubiquitous feature of many lung diseases and elicits cell-specific responses. While the effects of hypoxia on stem cells have been examined under in vitro conditions, the consequences of in vivo oxygen deprivation have not been studied. We investigated the effects of in vivo hypoxia on a recently characterized population of pluripotent stem cells known as very small embryonic-like stem cells (VSELs) by whole-genome expression profiling and measuring peripheral blood stem cell chemokine levels. We found that exposure to hypoxia in mice mobilized VSELs from the bone marrow to peripheral blood, and induced a distinct genome-wide transcriptional signature. Applying a computationally-intensive methodology, we identified a hypoxia-induced gene interaction network that was functionally enriched in a diverse array of programs including organ-specific development, stress response, and wound repair. Topographic analysis of the network highlighted a number of densely connected hubs that may represent key controllers of stem cell response during hypoxia and, therefore, serve as putative targets for altering the pathophysiologic consequences of hypoxic burden. A brief exposure to hypoxia recruits pluripotent stem cells to the p...Continue Reading

References

Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G L WangG L Semenza
Apr 29, 1993·The New England Journal of Medicine·T YoungS Badr
Apr 29, 1998·Clinics in Chest Medicine·S Redline, K P Strohl
Oct 16, 1999·Science·A L Barabasi, R Albert
Feb 22, 2001·Annual Review of Physiology·W V Cardoso
Mar 5, 2002·American Journal of Respiratory and Critical Care Medicine·Naresh M PunjabiPhilip L Smith
Apr 2, 2002·Nature Genetics·Jianhua ZhangMing Xu
May 7, 2002·American Journal of Respiratory and Critical Care Medicine·Terry YoungDaniel J Gottlieb
Mar 5, 2003·BioTechniques·A I SaeedJ Quackenbush
Mar 5, 2003·American Journal of Respiratory and Critical Care Medicine·Barry W RowDavid Gozal
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Nov 1, 2003·Sleep Medicine·Mary J MorrellDouglas R Corfield
Jan 22, 2004·Nature Reviews. Genetics·Albert-László Barabási, Zoltán N Oltvai
Apr 17, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Stephen R Reeves, David Gozal
Jul 6, 2004·Nature Medicine·Daniel J CeradiniGeoffrey C Gurtner
Sep 17, 2004·Nature·Nicholas M LuscombeMark Gerstein
Dec 2, 2004·The European Respiratory Journal·M AlchanatisA Papadimitriou
Jun 25, 2005·American Journal of Respiratory and Critical Care Medicine·Ellen L BurnhamMarc Moss
Jul 5, 2005·American Journal of Respiratory and Critical Care Medicine·Guanxia ZhanSigrid C Veasey
Sep 2, 2005·Nature·Steve E CalvanoUNKNOWN Inflamm and Host Response to Injury Large Scale Collab. Res. Program
Feb 4, 2006·Circulation Research·Levon M Khachigian
Mar 2, 2006·The European Respiratory Journal·P PalangeM R Bonsignore
Apr 8, 2006·Stem Cells·Gian Paolo FadiniCarlo Agostini
May 2, 2006·Archivum Immunologiae Et Therapiae Experimentalis·Magda KuciaMariusz Z Ratajczak
May 27, 2006·Molecular Systems Biology·Kanae OdaHiroaki Kitano
Sep 23, 2006·The New England Journal of Medicine·Volker SchächingerUNKNOWN REPAIR-AMI Investigators
Jan 5, 2007·Proceedings of the American Thoracic Society·Vsevolod Y Polotsky, Christopher P O'Donnell
Feb 3, 2007·Journal of Applied Physiology·Ah-Mee Park, Yuichiro J Suzuki
Feb 3, 2007·American Journal of Respiratory and Critical Care Medicine·Nao IiyoriChristopher P O'Donnell
Mar 8, 2007·American Journal of Respiratory and Critical Care Medicine·Kenji MinoguchiMitsuru Adachi
Apr 4, 2007·Cell Metabolism·Shaohui Huang, Michael P Czech

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Citations

Nov 28, 2012·Sleep & Breathing = Schlaf & Atmung·Macy Mei-Sze LuiMary Sau-Man Ip
May 4, 2011·Current Opinion in Hematology·Jonathan Hoggatt, Louis M Pelus
Aug 27, 2011·American Journal of Respiratory and Critical Care Medicine·Deepti NairDavid Gozal
Jan 17, 2012·Respiratory Physiology & Neurobiology·Theodora TsapikouniRamon Farré
Nov 25, 2011·Journal of Psychoactive Drugs·David E Smith
May 20, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Isaac AlmendrosDavid Gozal
Dec 20, 2019·Therapeutic Advances in Respiratory Disease·Abdelnaby KhalyfaDavid Gozal
Apr 6, 2021·Aging and Disease·Yayong Li, Yina Wang

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Methods Mentioned

BETA
fluorescence-activated cell sorting
FACS
ELISA

Software Mentioned

Gene Ontology analysis
Database for Annotation , Visualization and Integrated Discove...

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