Transcriptional organization and regulation of the Vibrio anguillarum heme uptake gene cluster

Gene
Susana MouriñoJorge H Crosa

Abstract

Vibrio anguillarum can utilize heme and hemoglobin as iron sources. Nine genes, huvA, huvZ, huvX, tonB1, exbB1, exbD1, huvB, huvC, huvD, encoding the proteins involved in heme transport and utilization, are clustered in a 10-kb region of chromosomal DNA. Reverse Transcriptase-PCR analysis demonstrated that the gene cluster is arranged into three transcriptional units: (1) huvA, (2) huvXZ, and (3) tonB1exbB1D1-huvBCD. Transcriptional start sites for each huvA, huvX, and tonB1 promoters were identified by primer extension analysis, and their respective -10 and -35 regions were shown to exhibit similarity to those of sigma70-recognized promoters. Expression from the three promoters, as analyzed by transcriptional fusions to a promoter less lacZ gene, was regulated by the iron concentration. Furthermore, analysis of the beta-galactosidase activities of these fusions in a V. anguillarum fur mutant demonstrated that the ferric uptake regulator repressor protein (Fur) is directly involved in the negative iron-mediated regulation of the heme uptake cluster.

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Citations

Sep 24, 2009·Chemical Reviews·Moriah Sandy, Alison Butler
Jan 11, 2011·Journal of Bacteriology·Julia FrunzkeMichael Bott
Jan 9, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Manuel L Lemos, Carlos R Osorio
Feb 9, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Claudia S López, Jorge H Crosa
Aug 16, 2011·Journal of Fish Diseases·I FransH Rediers
Jan 30, 2020·Journal of Applied Microbiology·M L Lemos, M Balado
Aug 10, 2017·Frontiers in Cellular and Infection Microbiology·Yingjie Li, Qingjun Ma

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