PMID: 8955323Dec 1, 1996Paper

Transcriptional organization and regulation of the dnaK and groE operons of Chlamydia trachomatis

Journal of Bacteriology
Ming TanJ N Engel

Abstract

The transcriptional organization and regulation of the dnaK and groE heat shock operons of Chlamydia trachomatis were studied and found to resemble those of the cognate operons of Bacillus subtilis and Clostridium acetobutylicum. The gene order is conserved (hrcA-grpE-dnaK), but no dnaJ homolog could be identified in this region. The dnaK operon was transcribed as a low-abundance polycistronic mRNA whose levels did not increase upon exposure to heat shock. In contrast, a more abundant 2.3-kb mRNA encoding only the dnaK sequence was detectable, and its steady-state level increased upon heat shock. The transcription initiation sites of the dnaK and groE operons were found to be preceded by sequences that resemble an Escherichia coli sigma70 consensus promoter. Upstream of each putative promoter is an inverted repeat sequence which resembles a similar element (CIRCE [controlling inverted repeat of chaperone expression]) found upstream of the dnaK and groE operons in at least 27 eubacterial species. In vitro transcription studies utilizing partially purified C. trachomatis RNA polymerase demonstrated that the regions containing the putative promoter elements of the dnaK and groE operons are functional, although heat shock-regulated...Continue Reading

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Citations

Jul 21, 1998·Annals of the New York Academy of Sciences·G Segal, E Z Ron
Mar 10, 2016·The ISME Journal·Marie de BarsyPatrick H Viollier
Feb 23, 1999·Molecular Microbiology·C GrandvaletP Mazodier
May 7, 2009·FEMS Microbiology Reviews·Peter A Lund
Nov 4, 2015·Journal of Bacteriology·Christopher J Rosario, Ming Tan
Aug 26, 2000·Journal of Bacteriology·C S Schaumburg, M Tan
Mar 6, 2003·Journal of Bacteriology·Karuna P KarunakaranRobert C Brunham
Aug 29, 2006·Journal of Bacteriology·Li ShenYou-Xun Zhang
Oct 24, 2006·Journal of Bacteriology·P Scott Hefty, Richard S Stephens
Jun 23, 2006·Infection and Immunity·Sanghamitra MukhopadhyayJames T Summersgill
May 20, 2004·Journal of Bacteriology·Adam C Wilson, Ming Tan
Nov 12, 2002·Journal of Bacteriology·Adam C Wilson, Ming Tan
Nov 28, 2007·Research in Microbiology·Brian GrechPeter Timms

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