PMID: 2108125Apr 1, 1990Paper

Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilis

Journal of Bacteriology
A R ZuberiG W Ordal

Abstract

A cloned chemotaxis operon has been characterized. Thirteen representative che mutations from different complementation groups were localized on the physical map by recombination experiments. The use of integration plasmids established that at least 10 of these complementation groups within this locus are cotranscribed. An additional three complementation groups may form part of the same transcript. The direction of transcription and the time of expression were determined from chromosomal che-lacZ gene fusions. The promoter was cloned and localized to a 3-kilobase fragment. Expression of beta-galactosidase from this promoter was observed primarily during the logarithmic phase of growth. Three-factor PBS1 cotransduction experiments were performed to order the che locus with respect to adjacent markers. The cheF141 mutation is 70 to 80% linked to pyrD1. This linkage is different from that reported previously (G. W. Ordal, D. O. Nettleton, and J. A. Hoch, J. Bacteriol. 154:1088-1097, 1983). The cheM127 mutation is 57% linked by transformation to spcB3. The gene order determined from all crosses is pyrD-cheF-cheM-spcB.

References

Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D N Arnosti, M J Chamberlin
Jun 1, 1985·Microbiological Reviews·P J Piggot, J A Hoch
Nov 1, 1985·Journal of Bacteriology·G W OrdalJ R Kirby
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Jan 24, 1981·Nucleic Acids Research·J MessingP H Seeburg
Jun 1, 1983·Journal of Bacteriology·F A FerrariJ A Hoch
Feb 1, 1981·Journal of Bacteriology·A H Ullah, G W Ordal
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen

❮ Previous
Next ❯

Citations

Jan 1, 1994·DNA Sequence : the Journal of DNA Sequencing and Mapping·M L KirschG W Ordal
Aug 12, 2017·Molecular Microbiology·Christine DiethmaierDavid Dubnau
Jan 1, 1992·Molecular Microbiology·D S Bischoff, G W Ordal
Sep 1, 1992·Molecular Microbiology·P B CarpenterG W Ordal
Jul 11, 1998·Journal of Bacteriology·W EstacioL M Márquez-Magaña
Apr 1, 1994·Journal of Bacteriology·L M Márquez-Magaña, M J Chamberlin
Apr 1, 1994·Journal of Bacteriology·L M Márquez-MagañaM J Chamberlin
Aug 1, 1994·Journal of Bacteriology·D BarillaA Galizzi
Jun 1, 1991·Journal of Bacteriology·P M HauserA Galizzi
Jun 15, 2021·Journal of Bacteriology·Andrew M PhillipsDaniel B Kearns
Dec 17, 2005·Genes & Development·Daniel B Kearns, Richard Losick

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.