Transcriptional pattern analysis of adrenergic immunoregulation in mice. Twelve hours norepinephrine treatment alters the expression of a set of genes involved in monocyte activation and leukocyte trafficking

Journal of Neuroimmunology
M Gruber-OlipitzK Schauenstein

Abstract

We investigated in vivo effects of norepinephrine (NE) on the transcription of 200 immunologically relevant genes in the mouse. Balb/c mice were s.c. implanted with NE containing retard tablets. Twelve hours later, splenic mRNA was prepared and hybridized onto cDNA microarrays containing the sequences of the major cytokines, their receptors and all CD-antigens of the mouse. Consistent results were obtained with a set of five genes: in the NE-treated animals four genes (CXCR4, VCAM1, IL-1R2, CD 14) were found 2-8 fold upregulated as compared to sham treated animals, whereas the gene for CCR3 was downregulated (< 0.5 fold). The findings were confirmed using quantitative reverse transcriptase Real Time PCR. These first results prove the usefulness of gene microarray technology towards transcription pattern analysis in neuroimmune interactions. Furthermore, they support the relevance of catecholamines in the regulation of leukocyte migration and the inflammatory response.

References

Nov 1, 1991·Journal of Neuroimmunology·I Rinner, K Schauenstein
Mar 1, 1993·Brain, Behavior, and Immunity·D R MurrayA S Maisel
Jun 1, 1996·Brain, Behavior, and Immunity·R J BenschopM Schedlowski
Feb 12, 1998·The New England Journal of Medicine·A D Luster
Oct 14, 1998·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·N IshiyamaK Hirokawa
Jun 26, 2001·Allergy·H S Haas, K Schauenstein
Dec 11, 2002·Biochemical Pharmacology·Valeria Ayelli EdgarAna María Genaro
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Angelika BierhausPeter P Nawroth
Mar 5, 2003·Brain, Behavior, and Immunity·Virginia M SandersAdam P Kohm
Apr 23, 2004·Trends in Immunology·Bernhard MoserPius Loetscher

❮ Previous
Next ❯

Citations

Nov 1, 2012·Cell Stress & Chaperones·Thomas J ZieziulewiczDavid A Lawrence
May 4, 2011·Current Opinion in Hematology·Jonathan Hoggatt, Louis M Pelus
Mar 23, 2011·Stem Cell Research & Therapy·Jonathan Hoggatt, Louis M Pelus
Mar 28, 2012·Stem Cell Reviews and Reports·Yichi Zhang, Bing Huang
Feb 24, 2016·Bulletin of Experimental Biology and Medicine·E G SkurikhinA M Dygai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.