Transcriptional precision and accuracy in development: from measurements to models and mechanisms

Development
Lital BentovimAngela H DePace

Abstract

During development, genes are transcribed at specific times, locations and levels. In recent years, the emergence of quantitative tools has significantly advanced our ability to measure transcription with high spatiotemporal resolution in vivo Here, we highlight recent studies that have used these tools to characterize transcription during development, and discuss the mechanisms that contribute to the precision and accuracy of the timing, location and level of transcription. We attempt to disentangle the discrepancies in how physicists and biologists use the term 'precision' to facilitate interactions using a common language. We also highlight selected examples in which the coupling of mathematical modeling with experimental approaches has provided important mechanistic insights, and call for a more expansive use of mathematical modeling to exploit the wealth of quantitative data and advance our understanding of animal transcription.

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Citations

Mar 30, 2018·PLoS Computational Biology·Timothy E SaundersPeter T C So
Dec 31, 2019·Evolution; International Journal of Organic Evolution·Gizem KalaySusan E Lott
Jun 13, 2020·Molecular Systems Biology·Içvara BarbierYolanda Schaerli
Nov 21, 2019·ELife·Claudia RacioppiLionel Christiaen
Jan 15, 2021·Nature Reviews. Genetics·Evgeny Z KvonZeba Wunderlich
Jan 22, 2021·Development·Jihyun Irizarry, Angelike Stathopoulos
Feb 3, 2021·Development·Gašper Tkačik, Thomas Gregor
May 20, 2020·Current Biology : CB·Conor J McClune, Michael T Laub
Jul 10, 2021·ELife·Jordan Yupeng XiaoAlistair N Boettiger

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Methods Mentioned

BETA
imaging techniques
Fluorescence
smFISH
fluorescence microscopy

Software Mentioned

smFISH

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