Transcriptional profiles of different states of cancer stem cells in triple-negative breast cancer

Molecular Cancer
Mingshan LiuSuling Liu

Abstract

Breast cancer stem cells (BCSCs) are thought to be responsible for tumor initiation, metastasis and relapse. Our group and others have described markers useful in isolating BCSCs just as aldehyde dehydrogenase positive (ALDH+) or CD24-CD44+. In fact, cells which simultaneously express both sets of markers have the highest tumor initiating capacity. Although the transcriptomic profile of cells expressing each BCSC marker alone has been reported, the profile of the most tumorigenic population expressing both sets of markers has not. Here we used the biomarker combination of ALDH and CD24/CD44 to sort four populations isolated from triple-negative breast cancer (TNBC) patient-derived xenografts, and performed whole-transcriptome sequencing on each population. We systematically compared the profiles of the three states of BCSCs (ALDH+CD24-CD44+, ALDH+non-CD24-CD44+ and ALDH-CD24-CD44+) to that of the differentiated tumor cells (ALDH-non-CD24-CD44+). For the first time, we compared the ALDH+CD24-CD44+ BCSCs with the other two BCSC populations. In ALDH+CD24-CD44+ BCSCs, we identified P4HA2, PTGR1 and RAB40B as potential prognostic markers, which were virtually related to the status of BCSCs and tumor growth in TNBC cells.

References

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Citations

Jul 19, 2018·Biomedicines·Judy S Crabtree, Lucio Miele
Oct 4, 2018·Current Stem Cell Research & Therapy·Payam ZarrintajMohammad Reza Saeb
Jul 26, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Chao HuangCungang Wu
Aug 8, 2020·Frontiers in Molecular Biosciences·Esta SterneckKuppusamy Balamurugan
Sep 21, 2018·Cancer Communications·Mengying TongQuentin Liu
Mar 21, 2019·Cancer Informatics·Zhonglin QuDaniel R Catchpoole
Jul 28, 2020·Advanced Science·Lina ZhaoBaowei Jiao
Jan 24, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wenyan FuShi Hu
Jan 29, 2021·Proceedings of the National Academy of Sciences of the United States of America·K J PientaR M Axelrod
Mar 7, 2021·International Journal of Molecular Sciences·Juliane StrietzJochen Maurer
Mar 20, 2021·Seminars in Cancer Biology·Rui ZhangSuling Liu
May 18, 2021·The Journal of Cell Biology·Erik S LinklaterRytis Prekeris
Jun 18, 2021·Journal of the American Chemical Society·Julie M GarlickAnna K Mapp
Jul 27, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Kanakaraju ManupatiAmitava Das
Aug 24, 2021·Frontiers in Pharmacology·Xing WangZufeng Guo

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Methods Mentioned

BETA
xenografts
activated cell sorting
PCA
PCR

Software Mentioned

GSEA
Cytoscape
ClueGO
DAVID
Gene Set Enrichment Analysis ( GSEA )

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