Transcriptional Profiles of SmWRKY Family Genes and Their Putative Roles in the Biosynthesis of Tanshinone and Phenolic Acids in Salvia miltiorrhiza

International Journal of Molecular Sciences
Haizheng YuZong-Suo Liang

Abstract

Salvia miltiorrhiza Bunge is a Chinese traditional herb for treating cardiovascular and cerebrovascular diseases, and tanshinones and phenolic acids are the dominated medicinal and secondary metabolism constituents of this plant. WRKY transcription factors (TFs) can function as regulators of secondary metabolites biosynthesis in many plants. However, studies on the WRKY that regulate tanshinones and phenolics biosynthesis are limited. In this study, 69 SmWRKYs were identified in the transcriptome database of S. miltiorrhiza, and phylogenetic analysis indicated that some SmWRKYs had closer genetic relationships with other plant WRKYs, which were involved in secondary metabolism. Hairy roots of S. miltiorrhiza were treated by methyl jasmonate (MeJA) to detect the dynamic change trend of SmWRKY, biosynthetic genes, and medicinal ingredients accumulation. Base on those date, a correlation analysis using Pearson's correlation coefficient was performed to construct gene-to-metabolite network and identify 9 SmWRKYs (SmWRKY1, 7, 19, 29, 45, 52, 56, 58, and 68), which were most likely to be involved in tanshinones and phenolic acids biosynthesis. Taken together, this study has provided a significant resource that could be used for furth...Continue Reading

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Citations

Jul 20, 2018·Applied Microbiology and Biotechnology·Mallappa Kumara SwamyAli Ghasemzadeh
Dec 6, 2019·International Journal of Molecular Sciences·Xiaobing WangLikai Chen
Dec 20, 2020·International Journal of Molecular Sciences·Bin ZhangJuane Dong
Apr 4, 2021·International Journal of Molecular Sciences·Xiuhong LiJuane Dong

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Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

Cluster
CLUSTALW
MEGA
Compute pI / Mw
HMMER
SPSS
Empower
MEME
ExPASy
BLAST

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