Transcriptional profiling and biological pathway analysis of human equivalence PCB exposure in vitro: indicator of disease and disorder development in humans

Environmental Research
Somiranjan GhoshSisir K Dutta

Abstract

Our earlier gene-expression studies with a Slovak PCBs-exposed population have revealed possible disease and disorder development in accordance with epidemiological studies. The present investigation aimed to develop an in vitro model system that can provide an indication of disrupted biological pathways associated with developing future diseases, well in advance of the clinical manifestations that may take years to appear in the actual human exposure scenario. We used human Primary Blood Mononuclear Cells (PBMC) and exposed them to a mixture of human equivalence levels of PCBs (PCB-118, -138, -153, -170, -180) as found in the PCBs-exposed Slovak population. The microarray studies of global gene expression were conducted on the Affymetrix platform using Human Genome U133 Plus 2.0 Array along with Ingenuity Pathway Analysis (IPA) to associate the affected genes with their mechanistic pathways. High-throughput qRT-PCR Taqman Low Density Array (TLDA) was done to further validate the selected 6 differentially expressed genes of our interest, viz., ARNT, CYP2D6, LEPR, LRP12, RRAD, TP53, with a small population validation sample (n=71). Overall, we revealed a discreet gene expression profile in the experimental model that resembled t...Continue Reading

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Citations

Jan 12, 2017·International Journal of Environmental Research and Public Health·Feng HePaul J Arciero
Aug 23, 2020·International Journal of Environmental Research and Public Health·Zarish NoreenSomiranjan Ghosh
Apr 19, 2020·Reviews on Environmental Health·Serena BianchiGuido Macchiarelli
Aug 21, 2021·Autism : the International Journal of Research and Practice·Salvador Marí-BausetMaría Morales Suárez-Varela

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