Transcriptional profiling and genes involved in acquired thermotolerance in Banana: a non-model crop

Scientific Reports
S M VidyaK V Ravishankar

Abstract

Banana is a non- model crop plant, and one of the most important crops in the tropics and sub tropics. Heat stress is the major abiotic stress affecting banana crop production because of its long growth period and is likely to become a threat due to global warming. To understand an acquired thermotolerance phenomenon at the molecular level, the RNA-seq approach was employed by adapting TIR method. A total of 136.38 million high quality reads were assembled. Differentially expressed genes under induction (I) was 3936, I + L was 2268 and lethal stress was 907 compared to control. Gene ontology and DGE analysis showed that genes related to heat shock factors, heat shock proteins, stress associated proteins, ROS scavenging, fatty acid metabolism, protein modification were significantly up regulated during induction, thus preparing the organism or tissue at molecular and cellular level for acquired thermotolerance. KEGG pathway analysis revealed the significant enrichment of pathways involved in protein processing, MAPK signaling and HSPs which indicates that these processes are conserved and involved in thermo tolerance. Thus, this study provides insights into the acquired thermotolerance phenomena in plants especially banana.

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Jodi CallwoodSathya Elavarthi

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Datasets Mentioned

BETA
PRJNA320030

Methods Mentioned

BETA
Chip
RNA-seq
protein modification
PCR
RNA seq
electrophoresis

Software Mentioned

TopHat
house
Blastx
Cufflinks
R
Annocript
Bowtie
Blast2GO
Bowtie2
Gene Chip

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