Transcriptional profiling of disease-induced host responses in bovine tuberculosis and the identification of potential diagnostic biomarkers.

PloS One
E Aranday-CortesH M Vordermeier

Abstract

Bovine tuberculosis (bTb) remains a major and economically important disease of livestock. Improved ante-mortem diagnostic tools would help to underpin novel control strategies. The definition of biomarkers correlating with disease progression could have impact on the rational design of novel diagnostic approaches for bTb. We have used a murine bTb model to identify promising candidates in the host transcriptome post-infection. RNA from in vitro-stimulated splenocytes and lung cells from BALB/c mice infected aerogenically with Mycobacterium bovis were probed with high-density microarrays to identify possible biomarkers of disease. In antigen-stimulated splenocytes we found statistically significant differential regulation of 1109 genes early (3 days) after infection and 1134 at a later time-point post-infection (14 days). 618 of these genes were modulated at both time points. In lung cells, 282 genes were significantly modulated post-infection. Amongst the most strongly up-regulated genes were: granzyme A, granzyme B, cxcl9, interleukin-22, and ccr6. The expression of 14 out of the most up-regulated genes identified in the murine studies was evaluated using in vitro with antigen-stimulated PBMC from uninfected and naturally inf...Continue Reading

References

Dec 1, 1993·The Journal of Experimental Medicine·J L FlynnB R Bloom
Sep 16, 1998·Clinical and Experimental Immunology·A D Howard, B S Zwilling
Sep 3, 1999·Clinical and Diagnostic Laboratory Immunology·H M VordermeierR G Hewinson
Jun 30, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·H M VordermeierR G Hewinson
Jul 21, 2001·Tuberculosis·P R Wood, S L Jones
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·D H CanadayW H Boom
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jan 3, 2006·Veterinary Microbiology·Charles ThoenIsabel de Kantor
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Euan LockhartJoAnne L Flynn
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas J ScribaWillem A Hanekom
Jan 26, 2008·Cytokine·Shabaana A Khader, Andrea M Cooper
Apr 19, 2008·The Journal of Infectious Diseases·Ajit Lalvani, Kerry A Millington
Apr 29, 2008·Current Opinion in Immunology·Christina L RoarkRebecca L O'Brien
Jun 28, 2008·Cellular & Molecular Immunology·Mei Yu PengBai Qing Li
Jul 2, 2008·Tuberculosis·Karen E LoganPhilip J Hogarth
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hiromasa HamadaRichard W Dutton
Mar 14, 2009·European Journal of Immunology·Rebecca L O'BrienWilli K Born
Apr 18, 2009·Microbes and Infection·Federica Sallusto, Antonio Lanzavecchia
Jun 3, 2009·Infection and Immunity·H Martin VordermeierR Glyn Hewinson
Oct 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rohan DhimanRamakrishna Vankayalapati
Nov 3, 2009·Immunity·Natalia Martin-OrozcoChen Dong
Mar 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuko Okamoto YoshidaGoro Matsuzaki
Mar 12, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mark S WilsonThomas A Wynn
Apr 8, 2010·Journal of Leukocyte Biology·Bridget VesoskyGillian Beamer
Jun 12, 2010·Immunological Reviews·Desiree A AnthonyMark J Smyth
Jun 12, 2010·Immunological Reviews·Judy Lieberman
Dec 28, 2010·Tuberculosis·Federico Carlos BlancoFabiana Bigi
Jan 5, 2011·Clinical & Developmental Immunology·W Ray WatersD Mark Estes
Jan 5, 2011·Clinical & Developmental Immunology·Carlos Rodrigo Zárate-BladésGeraldo A Passos
Jan 14, 2011·Natural Product Reports

❮ Previous
Next ❯

Citations

May 27, 2014·Veterinary Immunology and Immunopathology·Federico Carlos BlancoMarcelo Abel Soria
Feb 18, 2016·Annual Review of Animal Biosciences·H Martin VordermeierBernardo Villarreal-Ramos
Jan 22, 2013·Expert Opinion on Medical Diagnostics·Jason E McDermottKarin D Rodland
Apr 13, 2013·Methods : a Companion to Methods in Enzymology·K L FlanaganP Dickinson
Mar 26, 2016·Frontiers in Genetics·Mikolaj M RaszekGraham S Plastow
Aug 23, 2012·Transboundary and Emerging Diseases·E Aranday-CortesF J Salguero
Feb 22, 2012·Vaccine·W Ray WatersH Martin Vordermeier
Sep 11, 2014·Frontiers in Immunology·Kirsten E McLoughlinDavid E MacHugh
Dec 18, 2015·Clinical and Vaccine Immunology : CVI·W Ray WatersMichelle H Larsen
Mar 22, 2019·Veterinary Pathology·Mitchell V PalmerTyler C Thacker
Dec 12, 2012·PloS One·Alexander Churbanov, Brook Milligan
Sep 19, 2019·Infection and Immunity·Kathryn WrightKumudika de Silva
Feb 20, 2018·Experimental and Therapeutic Medicine·Qiu-Yue LiuZong-De Zhang
Oct 17, 2018·Frontiers in Microbiology·Aubrey BerryAdel M Talaat
Dec 29, 2020·Animal Health Research Reviews·Hyun-Eui Park, Han Sang Yoo
May 25, 2021·Frontiers in Veterinary Science·Mitchell V PalmerPaola M Boggiatto
Oct 12, 2016·Microbiology Spectrum·Bryce M BuddleR Glyn Hewinson
Aug 15, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rubina BunjunWendy A Burgers

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE33058

Methods Mentioned

BETA
FACS
ELISA
Fluorescence
PCR

Software Mentioned

Agilent
Prism Graph Pad
GeneSpring GX
Feature Software ( FES )

Related Concepts

Related Feeds

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.