Transcriptional profiling of endogenous germ layer precursor cells identifies dusp4 as an essential gene in zebrafish endoderm specification.

Proceedings of the National Academy of Sciences of the United States of America
Jamie L BrownBenjamin Feldman

Abstract

A major goal for developmental biologists is to define the behaviors and molecular contents of differentiating cells. We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This strategy enabled us to perform a genome-wide transcriptome comparison of germ layer precursor cells. Mesendoderm and ectoderm precursors cells isolated by this method differentiated appropriately in transplantation assays. Microarray analysis of these cells reidentified known genes at least as efficiently as previously reported strategies that relied on artificial mesendoderm activation or inhibition. We also identified a large set of uncharacterized mesendoderm-enriched genes as well as ectoderm-enriched genes. Loss-of-function studies revealed that one of these genes, the MAP kinase inhibitor dusp4, is essential for early development. Embryos injected with antisense morpholino oligonucleotides that targeted Dusp4 displayed necrosis of head tissues. Marker analysis during late gastrulation revealed a specific loss of sox17, but not of other endoderm markers, and analysis at later stages revealed a loss of foregut...Continue Reading

References

Jul 2, 1993·Science·R K Ho, C B Kimmel
Dec 19, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·T DickmeisU Strähle
Sep 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ryoko AndoAtsushi Miyawaki
Nov 8, 2002·Development·Laura S Gammill, Marianne Bronner-Fraser
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yutaka KikuchiDidier Y R Stainier
Nov 16, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Charles G SagerströmHazel Sive
Jul 29, 2005·Development·Staale EllingsenThomas S Becker
Aug 16, 2005·Molecular Endocrinology·Hyon J KimShuo Lin
Aug 20, 2005·Development, Growth & Differentiation·Kimiko Fukuda, Yutaka Kikuchi
Nov 16, 2005·Annual Review of Genetics·Alexander F Schier, William S Talbot
Apr 28, 2006·Journal of Cell Science·Vinzenz LinkCarl-Philipp Heisenberg
Sep 12, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Stephan A WackerWalter Knöchel
Feb 20, 2007·Developmental Biology·James T BennettAlexander F Schier
Mar 3, 2007·Developmental Biology·Richard I SherwoodDouglas A Melton
Apr 5, 2007·Nature Protocols·Kohei HattaTomomi Omura
Aug 28, 2007·Developmental Biology·Wuhong PeiBenjamin Feldman
Feb 12, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Christian SchröterAndrew C Oates

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Citations

Jan 27, 2009·Clinical & Experimental Metastasis·Lucia MartiniovaKarel Pacak
Jul 7, 2009·Nature Chemical Biology·Gabriela MolinaMichael Tsang
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Aaron M Zorn, James M Wells
Apr 29, 2010·BMC Developmental Biology·Sung-Kook HongBenjamin Feldman
May 26, 2011·BMC Developmental Biology·Liselotte VesterlundJuha Kere
Oct 31, 2012·BMC Medical Genomics·Peter M SzabóPeter Igaz
Mar 20, 2014·Toxicology and Applied Pharmacology·Ting XuQingshun Zhao
Nov 10, 2013·Disease Models & Mechanisms·Kandice FeroHarold A Burgess
Mar 14, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Milton A EnglishP Paul Liu
Jan 21, 2012·Biochemical Society Transactions·Ahmed LawanRobin Plevin
Feb 23, 2013·Molecular Plant·Heike WolfThorsten Seidel
May 14, 2010·American Journal of Physiology. Cell Physiology·O BermudezC Gimond
Sep 26, 2020·Frontiers in Cell and Developmental Biology·Jia YanHong Jiang

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