Transcriptional profiling of Giardia intestinalis in response to oxidative stress

International Journal for Parasitology
Showgy Y Ma'ayehStaffan G Svärd

Abstract

Giardia intestinalis is a microaerophilic parasite that infects the human upper small intestine, an environment that is fairly aerobic with reactive oxygen species being produced to fight off the parasite. It is quite perplexing how Giardia, lacking conventional eukaryotic antioxidant machinery (e.g. catalase, superoxide dismutase and glutathione peroxidase), can cope with the oxidative stress in this environment. We used transcriptomics (RNA sequencing and quantitative PCR) to study giardial gene expression changes in response to oxygen (O2; 1h) and hydrogen peroxide (H2O2; 150 μM, 500 μM and 1mM for 1h). The results showed phenotypic and transcriptional differences between Giardia isolates of different genotypes (WB, assemblage A and GS, assemblage B), with GS being more tolerant to H2O2 and exhibiting higher basic transcript levels of antioxidant genes (e.g. NADH oxidase lateral transfer candidate, peroxiredoxin 1 (Prx1) and thioredoxin (Trx)-like proteins). Cysteine is a major antioxidant in Giardia and its role in oxidative defense could be highlighted here by the up-regulation of gene transcripts encoding the cysteine-rich variable surface proteins (VSPs) and high cysteine membrane proteins (HCMPs). Genes in the thioredox...Continue Reading

References

Aug 1, 1990·Molecular and Biochemical Parasitology·T A PagetD Lloyd
Mar 1, 1990·American Journal of Surgery·W G SheridanH L Young
Jun 1, 1981·Experimental Parasitology·F D Gillin, L S Diamond
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·D B Keister
Jan 1, 1993·Molecular and Biochemical Parasitology·T A PagetD Lloyd
Mar 1, 1994·European Journal of Biochemistry·S M TownsonP Upcroft
Sep 1, 1993·Molecular and Biochemical Parasitology·D M BrownP Upcroft
Jun 1, 1995·Molecular and Biochemical Parasitology·D M BrownP Upcroft
Oct 1, 1996·European Journal of Biochemistry·D M BrownP Upcroft
Dec 20, 1996·Molecular and Biochemical Parasitology·D M BrownP Upcroft
Oct 6, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Y R Ortega, R D Adam
Mar 21, 1998·International Journal for Parasitology·D M BrownP Upcroft
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·G HeJ L Zweier
Jun 1, 2000·Free Radical Biology & Medicine·R T LightfootH Ischiropoulos
Dec 13, 2000·International Journal for Parasitology·R C Thompson
Nov 20, 2001·Nucleic Acids Research·H G ElmendorfT E Nash
Mar 8, 2002·Microbiology·David LloydJanine C Harris
Jul 12, 2002·Critical Reviews in Microbiology·Samantha Lane, David Lloyd
Dec 31, 2003·Molecular and Biochemical Parasitology·Md Ali AkbarPradeep Das
May 11, 2004·Microbiology·Timothy PagetDavid Lloyd
Mar 17, 2005·Cellular and Molecular Life Sciences : CMLS·M P Mayer, B Bukau
May 17, 2005·Progress in Lipid Research·Roberta LeonardiSuzanne Jackowski
Mar 21, 2006·Trends in Parasitology·L SavioliA Thompson
Apr 24, 2007·Free Radical Biology & Medicine·Diego G AriasSergio A Guerrero
Dec 14, 2007·The Journal of Biological Chemistry·Adele Di MatteoAlessandro Giuffrè
Dec 5, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·Roberta Ribeiro SilvaMariângela Carneiro
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
May 26, 2009·Molecular and Biochemical Parasitology·Virginie SauvageIsabelle Villena
Jun 24, 2009·Archives of Biochemistry and Biophysics·João B VicenteAlessandro Giuffrè
Jul 27, 2010·Biochemical and Biophysical Research Communications·Steven RaffertyJanet Yee
Aug 10, 2010·Biochemical and Biophysical Research Communications·Daniela MastronicolaAlessandro Giuffrè
Oct 26, 2010·Antioxidants & Redox Signaling·Andrea HallP Andrew Karplus
Nov 16, 2010·International Journal for Parasitology·Emma RingqvistStaffan G Svärd
Feb 23, 2011·International Journal of Molecular Sciences·Ralee Spooner, Ozlem Yilmaz
Jun 24, 2011·Bioinformatics·Adam RobertsLior Pachter
Aug 6, 2011·Current Protocols in Toxicology·Leslie B PooleKimberly J Nelson
Nov 8, 2011·Antioxidants & Redox Signaling·R Luise Krauth-Siegel, Alejandro E Leroux

❮ Previous
Next ❯

Citations

Dec 18, 2015·Molecular and Biochemical Parasitology·Daniela MastronicolaAlessandro Giuffrè
Feb 18, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alberto Guevara-FloresIrene Patricia Del Arenal
Sep 11, 2019·Genome Biology and Evolution·Alejandro Jiménez-GonzálezJan O Andersson
Oct 6, 2020·Frontiers in Genetics·Dimitra PeirasmakiStaffan G Svärd
Mar 14, 2018·The Korean Journal of Parasitology·Dibyendu RajSandipan Ganguly
Jun 18, 2017·Frontiers in Cellular and Infection Microbiology·Jonathan K PhamScott C Dawson
Sep 16, 2019·International Journal of Biological Macromolecules·Nikhilesh Joardar, Santi P Sinha Babu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.