Transcriptional Profiling of Host Cell Responses to Virulent Haemophilus parasuis : New Insights into Pathogenesis

International Journal of Molecular Sciences
Shulin FuChien-An Andy Hu

Abstract

Haemophilus parasuis is the causative agent of Glässer’s disease in pigs. H. parasuis can cause vascular damage, although the mechanism remains unclear. In this study, we investigated the host cell responses involved in the molecular pathway interactions in porcine aortic vascular endothelial cells (PAVECs) induced by H. parasuis using RNA-Seq. The transcriptome results showed that when PAVECs were infected with H. parasuis for 24 h, 281 differentially expressed genes (DEGs) were identified; of which, 236 were upregulated and 45 downregulated. The 281 DEGs were involved in 136 KEGG signaling pathways that were organismal systems, environmental information processing, metabolism, cellular processes, and genetic information processing. The main pathways were the Rap1, FoxO, and PI3K/Akt signaling pathways, and the overexpressed genes were determined and verified by quantitative reverse transcription polymerase chain reaction. In addition, 252 genes were clustered into biological processes, molecular processes, and cellular components. Our study provides new insights for understanding the interaction between bacterial and host cells, and analyzed, in detail, the possible mechanisms that lead to vascular damage induced b...Continue Reading

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Related Concepts

Metazoa
Endothelium, Vascular
Haemophilus Infections
Family suidae
Phosphatidylinositol 3-Kinases
Vascular Endothelial Cells
Haemophilus parasuis
RAC-Alpha Serine/Threonine Kinase
Forkhead Transcription Factors
Gene Expression Profiles

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