Transcriptional Profiling of Immune-Related Genes in Leishmania infantum -Infected Mice: Identification of Potential Biomarkers of Infection and Progression of Disease

Frontiers in Cellular and Infection Microbiology
Eduardo OntoriaEmma Carmelo

Abstract

Leishmania spp. is a protozoan parasite that affects millions of people around the world. At present, there is no effective vaccine to prevent leishmaniases in humans. A major limitation in vaccine development is the lack of precise understanding of the particular immunological mechanisms that allow parasite survival in the host. The parasite-host cell interaction induces dramatic changes in transcriptome patterns in both organisms, therefore, a detailed analysis of gene expression in infected tissues will contribute to the evaluation of drug and vaccine candidates, the identification of potential biomarkers, and the understanding of the immunological pathways that lead to protection or progression of disease. In this large-scale analysis, differential expression of 112 immune-related genes has been analyzed using high-throughput qPCR in spleens of infected and naïve Balb/c mice at four different time points. This analysis revealed that early response against Leishmania infection is characterized by the upregulation of Th1 markers and M1-macrophage activation molecules such as Ifng, Stat1, Cxcl9, Cxcl10, Ccr5, Cxcr3, Xcl1, and Ccl3. This activation doesn't protect spleen from infection, since parasitic burden rises along time. ...Continue Reading

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Citations

Oct 15, 2018·Immunology·René H M RaevenGideon F A Kersten
Oct 23, 2019·Vaccines·Shubhranshu ZutshiBhaskar Saha
Nov 16, 2018·Frontiers in Immunology·Fernanda Tomiotto-PellissierWander Rogério Pavanelli
Jun 20, 2019·Frontiers in Veterinary Science·Javier Moreno
May 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ehab Kotb ElmahallawyAmira A Saleh
Jul 21, 2021·International Journal of Molecular Sciences·Génesis PalaciosEmma Carmelo
Oct 19, 2021·Frontiers in Cellular and Infection Microbiology·Fernanda Tomiotto-PellissierWander Rogério Pavanelli

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Methods Mentioned

BETA
RNA-seq
PCR

Software Mentioned

qBase Plus
GraphPad Prism
SPSS
geNorm

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