Transcriptional profiling of initial differentiation events in human embryonic stem cells

Biochemical and Biophysical Research Communications
John D CalhounSteven L Stice

Abstract

Currently, there are no differentiation strategies for human embryonic stem cells (hESCs) that efficiently produce one specific cell type, possibly because of lack of understanding of the genes that control signaling events prior to overt differentiation. sed HepG2 cell conditioned medium (MEDII), which induces early differentiation in mouse ES cells while retaining pluripotent markers, to query gene expression in hESCs. Treatment of adherent hESCs with 50% MEDII medium effected differentiation to a cell type with gene expression similar to primitive streak stage cells of mouse embryos. MEDII treatment up-regulates TDGF1 (Cripto), a gene essential for anterior-posterior axis and mesoderm formation in mouse embryos and a key component of the TGFB1/NODAL signaling pathway. LEFTYA, an antagonist of NODAL/TDGF1 signaling expressed in anterior visceral endoderm, is down-regulated with MEDII treatment, as is FST, an inhibitor of mesoderm induction via the related INHBE1 pathway. In summary, the TGFB1/NODAL pathway is important for primitive-streak and mesoderm formation and in using MEDII, we present a means for generating an in vitro cell population that maintains pluripotent gene expression (POU5F1, NANOG) and SSEA-4 markers while ...Continue Reading

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Citations

Nov 30, 2005·Rejuvenation Research·Quan-Jian YanYun-Hui Zhang
May 2, 2006·Stem Cells and Development·Deanne TibbittsSteven L Stice
Sep 25, 2008·Stem Cells and Development·Suzanne D WestfallToshihiko Ezashi
Apr 19, 2012·Experimental Hematology·Melanie D Kardel, Connie J Eaves
Feb 2, 2010·Biochemical and Biophysical Research Communications·Sheena AbrahamRaj R Rao
Sep 7, 2007·Biochemical and Biophysical Research Communications·Ming ZhaoKaitai Yao
Oct 18, 2007·Journal of Cellular Physiology·Mario MancinoCaterina Bianco
Nov 28, 2007·Biochemical and Biophysical Research Communications·Bose S KochupurakkalDavid Givol
Oct 10, 2015·Journal of Tissue Engineering and Regenerative Medicine·Yunyi KangAthanasios Mantalaris
Jan 26, 2011·PloS One·Azhari AzizNaomi E Bishop
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Natanel Korin, Shulamit Levenberg
Sep 1, 2006·Biology of Reproduction·James A ByrneDon P Wolf
Sep 5, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Caterina BiancoNicola Normanno
Sep 24, 2005·Journal of Cell Science·Ludovic VallierRoger A Pedersen

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