Transcriptional profiling of mesenchymal stromal cells from young and old rats in response to Dexamethasone.

BMC Genomics
Uri David AkaviaDafna Benayahu

Abstract

Marrow-derived stromal cells (MSCs) maintain the capability of self-renewal and differentiation into multiple lineages in adult life. Age-related changes are recognized by a decline in the stemness potential that result in reduced regeneration potential of the skeleton. To explore the molecular events that underline skeletal physiology during aging we catalogued the profile of gene expression in ex vivo cultured MSCs derived from 3 and 15 month old rats. The ex vivo cultured cells were analyzed following challenge with or without Dexamethasone (Dex). RNA retrieved from these cells was analyzed using Affymetrix Gene Chips to compare the effect of Dex on gene expression in both age groups. The molecular mechanisms that underline skeletal senescence were studied by gene expression analysis of RNA harvested from MSCs. The analysis resulted in complex profiles of gene expression of various differentiation pathways. We revealed changes of lineage-specific gene expression; in general the pattern of expression included repression of proliferation and induction of differentiation. The functional analysis of genes clustered were related to major pathways; an increase in bone remodeling, osteogenesis and muscle formation, coupled with a d...Continue Reading

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Citations

Sep 11, 2008·Physiological Genomics·Uri David Akavia, Dafna Benayahu
Jul 3, 2010·Redox Report : Communications in Free Radical Research·H SoneT Fukuda
Oct 24, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Silvia PierottiAndrea M Isidori
May 2, 2008·Journal of Cellular Physiology·Uri David AkaviaDafna Benayahu
Jul 30, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Le LinGuang-Yu Fan

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Datasets Mentioned

BETA
GSE3339

Methods Mentioned

BETA
gene array
chip
electrophoresis
PCR

Software Mentioned

affy
MAS
EXPANDER
Bioconductor
GO Tree Machine ( GOTM )
TINA

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