Transcriptional profiling of peripheral blood cells in clinical pharmacogenomic studies

Pharmacogenomics
Michael E Burczynski, Andrew J Dorner

Abstract

Peripheral blood represents an attractive tissue source in clinical pharmacogenomic studies, given the feasibility of its collection from patients and its potential as a sentinel tissue to monitor perturbations of physiology in many disease states. The hypothesis is that the circulating blood cells monitor the physiological state of the organism and alter their transcriptome in response to this surveillance. However, the successful implementation of transcriptional profiling of peripheral blood cells in clinical trials represents a tremendous technical challenge for several reasons, including controlling the pre-analytical variables associated with sample processing and the interpretation of gene expression signatures generated from the complex mixture of cell types in blood. Multiple approaches for identifying transcriptomes in peripheral blood cells exist and each method is associated with significant advantages and disadvantages. Nonetheless, a growing number of studies are rapidly identifying transcriptional biomarkers in peripheral blood cells that may function as biomarkers of disease, evidence of pharmacodynamic effect, or even predictors of clinical outcomes and risk of toxicity. This review highlights the major approac...Continue Reading

References

Feb 28, 2001·Journal of Immunological Methods·C HärtelH Klüter
Jun 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin MaasThomas M Aune
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Emily C BaechlerTimothy W Behrens
Mar 7, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yang TangFrank R Sharp
May 10, 2003·Journal of Immunological Methods·Patrick StordeurMichel Goldman
Jun 19, 2003·Oncogene·Michal Oskar NowickiTomasz Skorski
Jul 23, 2003·Current Pharmaceutical Design·Thomas M AuneNancy J Olsen
Dec 19, 2003·Arthritis Research & Therapy·Mary K Crow, Jay Wohlgemuth
Jan 22, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lee Anne McLeanMarlene Dressman
Apr 16, 2004·The New England Journal of Medicine·Lars BullingerJonathan R Pollack
Apr 16, 2004·The New England Journal of Medicine·Peter J M ValkRuud Delwel
May 15, 2004·Arthritis Research & Therapy·Nancy J OlsenThomas M Aune
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Robert J FeezorJames M Seeger
Jun 4, 2004·Journal of Translational Medicine·Monica C PanelliFrancesco M Marincola
Jun 30, 2004·Journal of Neuroimmunology·Jian HongJingwu Z Zhang
Jul 1, 2004·The Journal of Nutrition·Karl B AndreeLiping Huang
Jul 9, 2004·British Journal of Haematology·Stephen BreitAndreas E Kulozik
Aug 17, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Stuart M FlechnerDaniel R Salomon
Aug 17, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Violeta RusCharles S Via
Aug 17, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Elizabeth E MannickZhiyun Liu
Oct 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kathleen H RubinsDavid A Relman
Oct 30, 2004·Current Opinion in Immunology·Emily C BaechlerTimothy W Behrens
Nov 19, 2004·Physiological Genomics·Robert J FeezorUNKNOWN Inflammation and Host Response to Injury, Large-Scale Collaborative Research Program
Dec 8, 2004·Brain Research. Molecular Brain Research·Yang TangFrank R Sharp
Jan 5, 2005·PLoS Biology·Sergio E BaranziniJorge R Oksenberg
Feb 16, 2005·Archives of Neurology·Yang TangFrank R Sharp

❮ Previous
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Citations

Sep 23, 2008·Molecular Diagnosis & Therapy·Anders Lönneborg
Feb 21, 2013·Pflügers Archiv : European journal of physiology·Paula OliverAndreu Palou
May 9, 2012·Attention Deficit and Hyperactivity Disorders·Edna GrünblattAngelika Schmitt
Dec 27, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ilan HalperinSilvia A Mandel
Nov 17, 2011·The British Journal of Nutrition·Antonio CamargoJose Lopez-Miranda
Feb 10, 2010·International Journal of Obesity : Journal of the International Association for the Study of Obesity·A CaimariA Palou
Aug 31, 2010·Nature Medicine·Kenneth T KotzUNKNOWN Inflammation and the Host Response to Injury Collaborative Research Program
Sep 19, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jose de Leon
Jun 2, 2010·Nature Reviews. Drug Discovery·Federico M GoodsaidIssam Zineh
Aug 3, 2010·The Pharmacogenomics Journal·J HuangP R Bushel
Dec 24, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Cynthia A AfshariPierre R Bushel
Jun 9, 2009·BMC Medical Genomics·Chris KarlovichJohn P Caulfield
Nov 23, 2012·Journal of Clinical Bioinformatics·Philip S CrookeThomas M Aune
Feb 1, 2008·Biomarkers in Medicine·Vanessa DumeauxAnne-Lise Børresen-Dale
Mar 7, 2006·Pharmacogenomics·Alison E Baird
May 19, 2010·Biomarkers in Medicine·Donna L Mendrick, Laura Schnackenberg
Mar 31, 2010·Pharmacogenomics·Yuxia Cui, Richard S Paules
Jul 25, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Antonio CamargoJosé López-Miranda
Sep 21, 2010·Biochemical and Biophysical Research Communications·Shruta S DadarkarMuralidhara Padigaru
Sep 23, 2014·Biological Trace Element Research·Hwayoung NohJayong Chung
Nov 9, 2007·Current Opinion in Allergy and Clinical Immunology·Li GaoKathleen C Barnes
Oct 9, 2015·International Journal of Environmental Research and Public Health·Cinzia La RoccaAlberto Mantovani
Dec 18, 2008·Expert Review of Neurotherapeutics·Edna Grünblatt
Oct 1, 2007·Expert Opinion on Medical Diagnostics·Enrico Domenici, Pierandrea Muglia
Sep 1, 2007·Expert Opinion on Drug Discovery·Silvia MandelMoussa Bh Youdim
Dec 3, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Carmen J SerranoJosé Antonio Enciso-Moreno
Sep 11, 2012·The Journal of Nutritional Biochemistry·Antonio CamargoJosé Lopez-Miranda
Nov 26, 2008·Journal of Pharmacological and Toxicological Methods·Johanna M BeekmanJürgen F Heubach
Oct 22, 2008·Toxicology Letters·Christine Debouck

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Methods Mentioned

BETA
biopsies
blood draw
Chip
fluorescent
activated cell sorting
chips
PCR

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