Transcriptional profiling reveals gene expression changes associated with inflammation and cell proliferation following short-term inhalation exposure to copper oxide nanoparticles

Journal of Applied Toxicology : JAT
Pedro M CostaBengt Fadeel

Abstract

Our recent studies revealed a dose-dependent proinflammatory response to copper oxide nanoparticles (CuO NPs) in rats following short-term inhalation exposure for five consecutive days. Here transcriptomics approaches were applied using the same model to assess global gene expression in lung tissues obtained 1 day post-exposure and after a recovery period of 22 days from rats exposed to clean air or 6 hour equivalent doses of 3.3 mg m-3 (low dose) and 13.2 mg m-3 (high dose). Microarray analyses yielded about 1000 differentially expressed genes in the high-dose group and 200 in low-dose compared to the clean air control group, and less than 20 after the recovery period. Pathway analysis indicated cell proliferation/survival and inflammation as the main processes triggered by exposure to CuO NPs. We did not find significant perturbations of pathways related to oxidative stress. Upregulation of epithelial cell transforming protein 2 (Ect2), a known oncogene, was noted and ECT2 protein was upregulated in the lungs of exposed animals. Proliferation of alveolar epithelial cells was demonstrated based on Ki67 expression. The gene encoding monocyte chemoattractant protein 1 (or CCL2) was also upregulated and this was confirmed by immu...Continue Reading

References

Jan 21, 1995·Lancet·A SeatonD Godden
Feb 1, 1996·Toxicology and Applied Pharmacology·K E DriscollG Oberdörster
Oct 29, 1998·Biochemical and Biophysical Research Communications·Y OhyamaR Nagail
Oct 2, 2003·Genome Biology·Douglas A HosackRichard A Lempicki
Feb 2, 2006·BMC Molecular Biology·Andreas SchroederThomas Ragg
Aug 20, 2008·Chemical Research in Toxicology·Hanna L KarlssonLennart Möller
Nov 4, 2008·Genetical Research·M Kathleen Kerr, Gary A Churchill
Jan 2, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daizaburo HirataYataro Daigo
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Aug 25, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Baher Fahmy, Stephania A Cormier
Apr 28, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura L KothPrescott G Woodruff
Sep 4, 2010·American Journal of Respiratory Cell and Molecular Biology·Jie ChaoNorberto C Gonzalez
Nov 5, 2010·BMC Research Notes·Nicolas ServantPhilippe Hupé
Feb 8, 2011·Toxicology and Applied Pharmacology·Vickie S WilsonSusan Y Euling
Jun 10, 2011·Nature·Bin-Zhi QianJeffrey W Pollard
Jul 29, 2011·Particle and Fibre Toxicology·Thomas Aj KuhlbuschMichael Stintz
Nov 10, 2012·The Science of the Total Environment·François PerreaultWilliam Gerson Matias
Nov 12, 2013·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Dec 18, 2013·Bioinformatics·Andreas KrämerStuart Tugendreich
Jan 23, 2014·Chemical Research in Toxicology·Shana J SturlaManuel C Peitsch
Jun 3, 2014·Particle and Fibre Toxicology·Pascal AndujarSophie Lanone
Jan 13, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Xuefang JingPeter S Thorne
Mar 22, 2015·The Annals of Occupational Hygiene·Mickael RinaldoPatrick Brochard
Jun 17, 2015·Epigenomics·Sukanya ShyamasundarBoon Huat Bay
Sep 24, 2016·International Journal of Nanomedicine·Nayana A PatilDeepti D Deobagkar
Apr 1, 2017·Chemical Research in Toxicology·Thomas HartungShana J Sturla

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Citations

Nov 30, 2019·International Journal of Environmental Research and Public Health·Carla MartinsPedro M Costa
Jan 15, 2020·IET Nanobiotechnology·Sania NazMuhammad Zia
Jul 7, 2019·Particle and Fibre Toxicology·Marit IlvesHarri Alenius
Apr 29, 2018·Scientific Reports·Anda R GligaHanna L Karlsson
Jul 1, 2020·Advanced Science·Artur Filipe RodriguesCyrill Bussy

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