Transcriptional profiling reveals molecular basis and novel genetic targets for improved resistance to multiple fermentation inhibitors in Saccharomyces cerevisiae

Biotechnology for Biofuels
Yingying ChenNa Wei

Abstract

Lignocellulosic biomass is a promising source of renewable biofuels. However, pretreatment of lignocellulosic biomass generates fermentation inhibitors that adversely affect the growth of industrial microorganisms such as Saccharomyces cerevisiae and prevent economic production of lignocellulosic biofuels. A critical challenge on developing S. cerevisiae with improved inhibitor resistance lies in incomplete understanding of molecular basis for inhibitor stress response and limited information on effective genetic targets for increasing yeast resistance to mixed fermentation inhibitors. In this study, we applied comparative transcriptomic analysis to determine the molecular basis for acetic acid and/or furfural resistance in S. cerevisiae. We recently developed a yeast strain YC1 with superior resistance to acetic acid, furfural, and their mixture through inverse metabolic engineering. In this study, we first determined transcriptional changes through RNA sequencing in YC1 versus the wild-type strain S-C1 under three different inhibitor conditions, including acetic acid alone, furfural alone, and mixture of acetic acid and furfural. The genes associated with stress responses of S. cerevisiae to single and mixed inhibitors were r...Continue Reading

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Citations

Mar 3, 2018·Applied Microbiology and Biotechnology·Nan XuLiming Liu
Apr 11, 2018·Microbial Cell Factories·Liang XiongXin-Qing Zhao
Sep 12, 2019·Biotechnology for Biofuels·Daria FeldmanOded Yarden
Jan 10, 2020·FEMS Yeast Research·Eun Joong Oh, Yong-Su Jin
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Oct 30, 2021·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Hyung Min BaeWon Hee Kang

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Methods Mentioned

BETA
chemical treatment
PCR
RNA-seq

Software Mentioned

Tophat2
YEASTRACT
DESeq2
package
DE
R
Btrim
Bowtie2
fastq
ea

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