Transcriptional Programs Driving Shear Stress-Induced Differentiation of Kidney Proximal Tubule Cells in Culture.

Frontiers in Physiology
Hyun Jung ParkOra A Weisz

Abstract

Cultured cell models are an essential complement to dissecting kidney proximal tubule (PT) function in health and disease but do not fully recapitulate key features of this nephron segment. We recently determined that culture of opossum kidney (OK) cells under continuous orbital shear stress (OSS) significantly augments their morphological and functional resemblance to PTs in vivo. Here we used RNASeq to identify temporal transcriptional changes upon cell culture under static or shear stress conditions. Comparison of gene expression in cells cultured under static or OSS conditions with a database of rat nephron segment gene expression confirms that OK cells cultured under OSS are more similar to the PT in vivo compared with cells maintained under static conditions. Both improved oxygenation and mechanosensitive stimuli contribute to the enhanced differentiation in these cells, and we identified temporal changes in gene expression of known mechanosensitive targets. We observed changes in mRNA and protein levels of membrane trafficking components that may contribute to the enhanced endocytic capacity of cells cultured under OSS. Our data reveal pathways that may be critical for PT differentiation in vivo and validate the utility ...Continue Reading

References

May 1, 1989·The American Journal of Physiology·A SahaiR L Tannen
Jul 1, 1986·The American Journal of Physiology·K Malmström, H Murer
Aug 1, 1986·The American Journal of Physiology·L A ColeR L Tannen
Aug 20, 1987·Biochimica Et Biophysica Acta·K MalströmH Murer
Aug 1, 1996·The American Journal of Physiology·M GekleS Silbernagl
Sep 24, 1999·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·G GstraunthalerW Pfaller
Oct 4, 2000·Physiological Reviews·H MurerJ Biber
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Oct 29, 2010·American Journal of Physiology. Renal Physiology·Zhenjie ZhuangDennis Brown
Apr 1, 2012·Comprehensive Physiology·Erik Ilsø ChristensenBrigitte Kaissling
Jun 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Venkatesan RaghavanOra A Weisz
Mar 31, 2015·Journal of the American Society of Nephrology : JASN·Jae Wook LeeMark A Knepper
Mar 11, 2016·Journal of the American Society of Nephrology : JASN·Stephen G SzetoDarren A Yuen
Nov 5, 2016·Annual Review of Physiology·Megan L Eshbach, Ora A Weisz
Jun 16, 2017·American Journal of Physiology. Renal Physiology·Megan L EshbachOra A Weisz
Jul 20, 2017·Molecular Biology of the Cell·Kimberly R LongOra A Weisz
Sep 6, 2017·The International Journal of Biochemistry & Cell Biology·Anuradha SubramanianNeety Sahu
Oct 19, 2017·Journal of Cellular Physiology·Steven J KunnenDorien J M Peters
Jan 25, 2018·Immunology and Cell Biology·Yuan ZhangQian Wang
Mar 4, 2019·Trends in Pharmacological Sciences·Niu NiuZheng-Gen Jin
Feb 19, 2020·American Journal of Physiology. Renal Physiology·Kimberly R LongOra A Weisz
Mar 24, 2020·American Journal of Physiology. Renal Physiology·Qidong RenOra A Weisz
Apr 3, 2020·Nature Reviews. Molecular Cell Biology·Isabell Bludau, Ruedi Aebersold
May 3, 2020·Journal of the American Society of Nephrology : JASN·Kavee LimbutaraMark A Knepper

❮ Previous
Next ❯

Citations

Apr 19, 2021·Current Opinion in Insect Science·Damandeep KapoorDennis Kolosov
May 27, 2021·The Journal of Physiology·Ora A Weisz
Jul 14, 2021·Journal of the American Society of Nephrology : JASN·Ora A Weisz
Oct 12, 2021·American Journal of Physiology. Renal Physiology·Shivangi AgarwalMehmet M Altintas

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE155315

Methods Mentioned

BETA
RNASeq
RNA Seq
Fluorescence
MDS
RNA-Seq

Key Resources (RRID) Mentioned

CVCL_0472

Software Mentioned

RNASeq
IPA
TopHat2
cutadapt
edgeR
GENCODE
Ingenuity Pathway Analysis ( IPA )
Subread package feature Counts
UCSC browser
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.