Feb 25, 2014

Transcriptional properties and splicing of the flamenco piRNA cluster

EMBO Reports
Coline GoriauxEmilie Brasset

Abstract

In Drosophila, the piRNA cluster, flamenco, produces most of the piRNAs (PIWI-interacting RNAs) that silence transposable elements in the somatic follicle cells during oogenesis. These piRNAs are thought to be processed from a long single-stranded precursor transcript. Here, we demonstrate that flamenco transcription is initiated from an RNA polymerase II promoter containing an initiator motif (Inr) and downstream promoter element (DPE) and requires the transcription factor, Cubitus interruptus. We show that the flamenco precursor transcript undergoes differential alternative splicing to generate diverse RNA precursors that are processed to piRNAs. Our data reveal dynamic processing steps giving rise to piRNA cluster precursors.

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Mentioned in this Paper

Stan protein, Drosophila
Nested Transcripts
RNA Polymerase II
PIWIL1 gene
Drosophila melanogaster Proteins
Elbow
Genes, Reiterated
Transcription, Genetic
Promoter
Drosophila

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