Transcriptional regulation and biological functions of selenium-binding protein 1 in colorectal cancer in vitro and in nude mouse xenografts.

PloS One
Nicole M PohlWancai Yang

Abstract

It has been shown that selenium-binding protein 1 (SBP1) is significantly downregulated in different human cancers. Its regulation and function have not yet been established. We show that the SBP1 promoter is hypermethylated in colon cancer tissues and human colon cancer cells. Treatment with 5'-Aza-2'-deoxycytidine leads to demethylation of the SBP1 promoter and to an increase of SBP1 promoter activity, rescues SBP1 mRNA and protein expression in human colon cancer cells. Additionally, overexpression of SBP1 sensitizes colon cancer cells to H2O2-induced apoptosis, inhibits cancer cell migration in vitro and inhibits tumor growth in nude mice. These data demonstrate that SBP1 has tumor suppressor functions that are inhibited in colorectal cancer through epigenetic silencing.

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Citations

Apr 4, 2014·Inflammatory Bowel Diseases·Bodo Speckmann, Holger Steinbrenner
Dec 15, 2010·Diagnostic Pathology·Peng ZhangW Dwayne Lawrence
May 3, 2014·International Journal of Molecular Sciences·Zhendong GuoLinna Liu
Nov 26, 2015·International Journal of Molecular Sciences·Qi YingWancai Yang
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Sep 25, 2017·Oncotarget·Shabarni GuptaSanjeeva Srivastava
Jun 9, 2020·The Prostate·Mostafa ElhodakyAlan M Diamond
Mar 14, 2013·International Journal of Oncology·Chenjing ZhangUndurti N Das
Dec 15, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Yi Yu-RiceJudith L Luborsky
Mar 15, 2018·Oncotarget·Mandy SchottSilke Kuphal
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Jul 14, 2018·Molecular Cancer Research : MCR·Deborah R CaswellMonte M Winslow

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Methods Mentioned

BETA
transfection
PCR
flow cytometry
xenografts
xenograft

Software Mentioned

methylation

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