Transcriptional regulation by transforming growth factor beta of the expression of retinoic acid and retinoid X receptor genes in osteoblastic cells is mediated through AP-1.

The Journal of Biological Chemistry
Y ChenS Hanazawa

Abstract

We now report that transforming growth factor beta1 (TGF-beta1), a potent regulatory cytokine of bone remodeling, is a powerful stimulator for gene expression of retinoic acid receptors (RARs) and retinoid X receptors (RXRs) in osteoblastic MC3T3-E1 cells. TGF-beta1 transcriptionally stimulated the expression of RARalpha, RARgamma, and RXRalpha genes, but did not do so for RARbeta, RXRbeta, and RXRgamma genes. We also observed that AP-1, a transcriptional factor, plays an important role in the signal pathway for expression of RARalpha, RARgamma, and RXRalpha genes stimulated by TGF-beta1 because stimulation of the expression of these genes in the cytokine-treated cells was markedly inhibited by a mixture of antisense c-fos and c-jun. A gel mobility shift assay demonstrated that TGF-beta1 is able to increase, in a dose-dependent manner, the binding of nuclear proteins to direct repeat 5, a consensus sequence with high affinity for RAR-RXR heterodimers. The mobility shift assay, using specific antibody for each receptor, showed that direct repeat 5-binding proteins may be RAR and RXR isoforms. The stimulated binding to direct repeat 5 was inhibited strongly by H-7, an inhibitor of serine/threonine kinase, and by curcumin, an inhi...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S A KliewerR M Evans
Dec 18, 1992·Science·J M LehmannM Pfahl
Oct 1, 1991·The Journal of Cell Biology·C Rochette-EglyP Chambon
May 1, 1991·Molecular and Cellular Biology·L J SuvaG A Rodan
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T S HuangJ K Lin
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A KukitaG D Roodman
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C ChenuG D Roodman
Aug 1, 1988·The Journal of Clinical Investigation·J PfeilschifterG R Mundy
Mar 1, 1981·Molecular and Cellular Biology·M GroudineH Weintraub
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T R BreitmanS J Collins
Aug 4, 1994·Nature·J L WranaJ Massagué
Jan 1, 1994·Progress in Growth Factor Research·P ten DijkeK Miyazono
May 21, 1993·Cell·D LohnesP Chambon

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Citations

Nov 21, 1998·Microscopy Research and Technique·N J HickokR S Tuan
Jul 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Alevizopoulos, N Mermod
Feb 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·C M KlingeR Hilf
Jul 25, 1998·Genes & Development·Y G ChenJ Massagué
Oct 6, 1998·Annual Review of Biochemistry·J Massagué
Sep 22, 2007·American Journal of Respiratory Cell and Molecular Biology·Anne DruilheMarina Pretolani
Jun 7, 2007·Journal of Cell Science·Xiaohong TanXiao Yang
Dec 29, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S PalcyD Goltzman
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P-L Wang, K Ohura
Aug 18, 2004·Biochemical and Biophysical Research Communications·A JacobsonH Melhus
Oct 26, 2007·Journal of Dental Research·T MinamiS Sugawara
Nov 30, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Katsuaki KanbeQian Chen
Nov 2, 2007·Nutrition Reviews·Judy D Ribaya-Mercado, Jeffrey B Blumberg
Mar 16, 2006·Journal of Cell Science·Sébastien VankoningslooThierry Arnould
Mar 26, 1998·Biochemical and Biophysical Research Communications·J J PotterF A Anania
Sep 27, 2007·Molecular Cancer·Jean-Philippe CosseCarine Michiels

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