Transcriptional Regulation, Metal Binding Properties and Structure of Pden1597, an Unusual Zinc Transport Protein from Paracoccus denitrificans.

The Journal of Biological Chemistry
Melody HandaliErik T Yukl

Abstract

ATP-binding cassette (ABC) transporters of the cluster 9 family are ubiquitous among bacteria and essential for acquiring Zn(2+) and Mn(2+) from the environment or, in the case of pathogens, from the host. These rely on a substrate-binding protein (SBP) to coordinate the relevant metal with high affinity and specificity and subsequently release it to a membrane permease for translocation into the cytoplasm. Although a number of cluster 9 SBP structures have been determined, the structural attributes conferring Zn(2+) or Mn(2+) specificity remain ambiguous. Here we describe the gene expression profile, in vitro metal binding properties, and crystal structure of a new cluster 9 SBP from Paracoccus denitrificans we have called AztC. Although all of our results strongly indicate Zn(2+) over Mn(2+) specificity, the Zn(2+) ion is coordinated by a conserved Asp residue only observed to date as a metal ligand in Mn(2+)-specific SBPs. The unusual sequence properties of this protein are shared among close homologues, including members from the human pathogens Klebsiella pneumonia and Enterobacter aerogenes, and would seem to suggest a subclass of Zn(2+)-specific transporters among the cluster 9 family. In any case, the unusual coordinati...Continue Reading

References

Nov 1, 1995·Scandinavian Journal of Immunology·P A Clohessy, B E Golden
Jul 15, 1999·Nature Structural Biology·Y H LeeC A Hasemann
Mar 14, 2000·Acta Crystallographica. Section D, Biological Crystallography·W P Burmeister
Jun 26, 2001·Research in Microbiology·J P Claverys
Apr 30, 2003·Journal of Structural Biology·Joseph R LuftGeorge T DeTitta
Jun 18, 2003·Biochemistry·Yongting WangVictor L Davidson
Oct 30, 2003·Journal of Molecular Biology·Soojay BanerjeeThomas J Smith
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Apr 22, 2005·Journal of Molecular Biology·Valeria RukhmanNoam Adir
Dec 21, 2006·Biochemistry·Misha V GolynskiySeth M Cohen
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Nov 21, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Liliya A YatsunykAmy C Rosenzweig
Mar 8, 2008·Acta Crystallographica. Section D, Biological Crystallography·Ivan DokmanićSanja Tomić
Jul 8, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Claudia AndreiniJanet M Thornton
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Nov 10, 2009·Methods in Enzymology·Petr Kuzmič
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·T Geoff G BattyeAndrew G W Leslie
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
Jul 26, 2011·Journal of Bacteriology·Paul J Wichgers SchreurHilde E Smith
Aug 9, 2011·Methods : a Companion to Methods in Enzymology·Paul D AdamsPeter H Zwart
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Nov 11, 2011·PLoS Pathogens·Christopher A McDevittJames C Paton
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Steven M DamoWalter J Chazin
Mar 23, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Margarita Kanteev, Noam Adir

❮ Previous
Next ❯

Citations

Oct 21, 2016·Biochimica Et Biophysica Acta·Nidhi SharmaAshwani Kumar Sharma
Sep 10, 2017·The Journal of Biological Chemistry·Durga P NeupaneErik T Yukl
Apr 27, 2017·Frontiers in Microbiology·Durga P NeupaneErik T Yukl
Aug 21, 2019·Communications Biology·Durga Prasad NeupaneErik Thomas Yukl
Jul 12, 2017·Acta Crystallographica. Section D, Structural Biology·Christopher D RadkaStephen G Aller
Dec 4, 2020·International Journal of Molecular Sciences·Ady Berenice MeléndezErik Thomas Yukl
Jan 12, 2020·Communications Biology·Durga Prasad NeupaneErik Thomas Yukl
Sep 8, 2021·Frontiers in Cellular and Infection Microbiology·Marina L ŽupanChristopher A McDevitt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.