Transcriptional regulation of an evolutionary conserved intergenic region of CDT2-INTS7.

PloS One
Hiroki NakagawaKenichi Yoshida

Abstract

In the mammalian genome, a substantial number of gene pairs (approximately 10%) are arranged head-to-head on opposite strands within 1,000 base pairs, and separated by a bidirectional promoter(s) that generally drives the co-expression of both genes and results in functional coupling. The significance of unique genomic configuration remains elusive. Here we report on the identification of an intergenic region of non-homologous genes, CDT2, a regulator of DNA replication, and an integrator complex subunit 7 (INTS7), an interactor of the largest subunit of RNA polymerase II. The CDT2-INTS7 intergenic region is 246 and 245 base pairs long in human and mouse respectively and is evolutionary well-conserved among several mammalian species. By measuring the luciferase activity in A549 cells, the intergenic human sequence was shown to be able to drive the reporter gene expression in either direction and notably, among transcription factors E2F, E2F1 approximately E2F4, but not E2F5 and E2F6, this sequence clearly up-regulated the reporter gene expression exclusively in the direction of the CDT2 gene. In contrast, B-Myb, c-Myb, and p53 down-regulated the reporter gene expression in the transcriptional direction of the INTS7 gene. Overex...Continue Reading

References

Feb 3, 1995·The Journal of Biological Chemistry·A Gavalas, H Zalkin
Jan 7, 2004·Genome Research·Nathan D TrinkleinRichard M Myers
Mar 3, 2004·Oncogene·Kenichi Yoshida, Ituro Inoue
Sep 15, 2006·Journal of Cellular Physiology·Yuya GotoKenichi Yoshida
Nov 7, 2006·Genes & Development·Christopher L SansamJacqueline A Lees
Nov 15, 2006·Current Molecular Medicine·David G Johnson, James Degregori
Mar 3, 2007·DNA and Cell Biology·Reiko HayashiKenichi Yoshida

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Citations

Mar 28, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rachael J Rutkowski, William D Warren
Dec 25, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S A B Garcia, M A Nagai
Mar 12, 2010·The Journal of Microbiology·Yi-Peng XuChuan-Xi Zhang
Feb 5, 2015·The Journal of Biological Chemistry·Veneta GerganovaGeorgi Muskhelishvili

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Methods Mentioned

BETA
transfection
PCR
Assay

Software Mentioned

CLUSTAL W
Transfac

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