Transcriptional regulation of cardiac progenitor cell populations

Circulation Research
Amanda MasinoDaniel J Garry

Abstract

Transcriptome-wide analysis of dynamically regulated progenitor cell populations has the potential to elucidate key aspects of cardiac development. The heart, as the first organ to develop in the mammal, is a technically challenging but clinically relevant target for study. To define the transcriptional program of the cardiac progenitor, we used a novel transgenic strategy and fluorescence-activated cell sorting to reliably label and isolate cardiac progenitors directly from mouse embryos. Pure populations of cardiac progenitor cells were isolated from the cardiac crescent and 2 subsequent stages of heart development: the linear heart tube and the looping heart. RNA was isolated from stage-specific cardiac progenitors and subjected to transcriptome analysis by oligonucleotide array hybridization. The cardiac transcriptional regulatory programs were compared with the molecular programs of age-matched noncardiac embryonic cells, embryonic stem cells, adult cardiomyocytes, and each other to identify sets of genes exhibiting differential expression in the cardiac progenitor cell population. These results define the transcriptional profile of mammalian cardiac progenitor cells and provide insight into the molecular regulation of the...Continue Reading

References

Jul 1, 1993·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P S Chan-ThomasP J Barton
Feb 15, 1997·Genes & Development·T M SchultheissA B Lassar
Mar 21, 1998·Genes & Development·J E VisvaderS H Orkin
Feb 13, 2001·Genes & Development·E Tzahor, A B Lassar
Feb 13, 2001·Genes & Development·M J MarvinA B Lassar
Jan 11, 2002·Developmental Biology·C H MjaatvedtR R Markwald
Feb 16, 2002·Journal of Hematotherapy & Stem Cell Research·Kyunghee Choi
Jun 21, 2002·Circulation Journal : Official Journal of the Japanese Circulation Society·Yuichi IkedaIssei Komuro
Jul 3, 2002·Nature Reviews. Genetics·Richard P Harvey
Oct 31, 2002·Development·Yun Shin ChungKyunghee Choi
Oct 11, 2003·Current Opinion in Genetics & Development·Giulio Cossu, Paolo Bianco
Nov 5, 2003·Genesis : the Journal of Genetics and Development·Teresa D GallardoDaniel J Garry
Apr 10, 2004·Science·Jennifer Couzin, Gretchen Vogel

❮ Previous
Next ❯

Citations

Feb 6, 2010·Pediatric Cardiology·Ian S Harris, Brian L Black
Jun 19, 2010·Journal of Cardiovascular Translational Research·Hesham A SadekDaniel J Garry
Mar 7, 2013·Nature Communications·Haruko NakanoAtsushi Nakano
Jan 19, 2007·Nature Clinical Practice. Cardiovascular Medicine·Beatriz PelachoFelipe Prósper
Dec 1, 2011·Nature Methods·Timothy J Kamp
Jan 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anwarul FerdousDaniel J Garry
Mar 13, 2013·Stem Cells and Development·Ann N BehrensCindy M Martin
Apr 28, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Dorien A M van DartelAldert H Piersma
Mar 24, 2005·Journal of Cellular and Molecular Medicine·Anke M SmitsPieter A Doevendans
Feb 5, 2011·Circulation Research·Anthony C Sturzu, Sean M Wu
Mar 31, 2012·Circulation Research·Ying Tian, Edward E Morrisey
Feb 5, 2008·The Journal of Clinical Investigation·Nicolas ChristoforouJohn D Gearhart
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yu LiuMichael D Schneider
May 10, 2007·Canadian Journal of Physiology and Pharmacology·Nichole M McMullen, Kishore B S Pasumarthi
Feb 2, 2016·Biochimica Et Biophysica Acta·Atsushi NakanoNathan J Palpant
Aug 14, 2012·Biochemical and Biophysical Research Communications·Takahiko NishiyamaKeiichi Fukuda
Jul 16, 2008·Journal of Cellular and Molecular Medicine·Nichole M McMullenKishore B S Pasumarthi
Sep 10, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Zachary NeebSimon J Conway
Sep 17, 2005·Experimental Cell Research·Martina StaryGeorg Weitzer
Mar 27, 2007·Differentiation; Research in Biological Diversity·Manuela HofnerGeorg Weitzer
Apr 20, 2006·Mechanisms of Development·Alexander AlexandrovichAlison C Brewer
Sep 4, 2012·American Journal of Human Genetics·Rachel SoemediBernard D Keavney
Nov 8, 2011·Developmental Biology·William SchachterleBrian L Black
Dec 19, 2006·Cell·Daniel J Garry, Eric N Olson
Aug 24, 2004·Circulation Research·Radwan Abu-Issa, Margaret L Kirby
Mar 17, 2007·The EMBO Journal·Annette P MeesonDaniel J Garry
Dec 26, 2006·Expert Review of Cardiovascular Therapy·Patrick van VlietMarie-José Goumans
Mar 1, 2007·Physiological Genomics·R Haris NaseemDaniel J Garry
Mar 12, 2020·Journal of Cardiovascular Development and Disease·Rusty Lansford, Sandra Rugonyi
Apr 16, 2020·Circulation Research·Ozanna Burnicka-TurekIvan P Moskowitz
Apr 13, 2021·Frontiers in Bioengineering and Biotechnology·Wangping ChenJianyi Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.